新疆马铃薯甲虫对噻虫嗪的抗性监测及其细胞色素P450基因表达分析

石鑫,李莎,王志敏,付开赟,付文君,姜卫华

Resistance Monitoring to Thiamethoxam and Expression Analysis of Cytochrome P450 Genes in Leptinotarsa decemlineata from Xinjiang

SHI Xin,LI Sha,WANG ZhiMin,FU KaiYun,FU WenJun,JIANG WeiHua

表2 马铃薯甲虫实时荧光定量PCR引物

Table 2 The primers ofL. decemlineata for qRT-PCR

基因 Gene 上游引物序列 Forward primer sequence (5′-3′) 下游引物序列 Reverse primer sequence (5′-3′) CYP9Ya TGGACCAAAGAGTACCCGAAGGAA TCGACAATTCAGTCCGAGGTACGA CYP9Yc ATTTGGAAGTGGACCACGCAATTG GCCAAATTCCTTCTCGTCCAGCAAG CYP9Z25 ACTTGCACACCTTTGAGAGCATCC TTGTCAACTTTCTCATGCCCACCC CYP4C1 AGCCTGACATTCCACTTC CTGCTCCTCACTAACATCT CYP9e2 CATCATAGCACAAGCATTCT TTTCCTCCACATATTTCATCAG CYP305a1 TTCCGAAGAATACGATGGT GCGAAGATGAAGAGTTACAA RPL4 AAAGAAACGAGCATTGCCCTTCCG TTGTCGCTGACACTGTAGGGTTGA Ef1α AAGGTTCCTTCAAGTATGCGTGGG GCACAATCAGCTTGCGATGTACCA