谷子条纹叶突变体A36-S的细胞学特性分析及基因定位
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张硕,智慧,唐婵娟,罗明昭,汤沙,贾冠清,贾彦超,刁现民
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Cytological Characters Analysis and Low-Resolution Mapping of Stripe-Leaf MutantA36-S in Foxtail Millet
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ZHANG Shuo,ZHI Hui,TANG ChanJuan,LUO MingZhao,TANG Sha,JIA GuanQing,JIA YanChao,DIAO XianMin
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表4 基因定位所用的标记及交换单株数量
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Table 4 Markers used in gene mapping and number of recombinant individuals
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标记名称
Marker name | 位置
Location (bp) | 引物序列
Primer sequence (5’-3’) | 交换株数量
Number of recombinant individuals | | In4-3 | 2485441 | F：GTATTCCCATGTTCGACGCGC
R：GTCGGTTATTAGTTGACTTGTTGTG | 167 | | InDel 14[25] | 4232606 | F：TACTCATTGCATCCCCTTCAGCAGC
R：CACTGGATAACGCATGGACTGACTA | 105 | | In4-17 | 6847221 | F：TGATGCCTTACTCCAGATTTCCAGC
R：GATGCATACTCAATACTCTCTAGCA | 26 | | In4-20 | 7661828 | F：GACTGTGAGGAGCCGCGTGTAC
R：GGCTCTCTCCCCTAACCGGC | 1 | | In4-21 | 7873802 | F：TGCTTCCCCAAATCAGTCTTTTAGT
R：GGGACACCTTAGAAACAAAACCAGA | 0 | | In4-29 | 23538740 | F：AGAAGGACTGCTTTGATCTGGC
R：GCCGCACACCATTTTGCATTACCT | 0 | | In4-32 | 27896330 | F：GCAATCGTCGTACACTACTCCA
R：GTAGCCAAGTGGTCATTGTTTT | 4 | | In4-33 | 30243701 | F：GGTTGTCTTGTGTGTTCT
R：GACAGGCTTCAAGATAAG | 15 | | SiCAAS4034[31] | 30398635 | F：CGGTGGGGTACATCGATCCTGCGACAT
R：TTTTCCCTTGAATGGCACGCTGCCGGG | 18 |
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