小麦单位面积穗数和粒长主效QTL紧密连锁KASP标记的开发及其效应评价

范涛,李治,蒋庆,陈姝霖,欧霞,陈永艳,任天恒

Development and Effect Evaluation of KASP Markers Closely Linked to Major QTLs of Spike Number Per Unit Area and Grain Length in Wheat

FAN Tao,LI Zhi,JIANG Qing,CHEN ShuLin,OU Xia,CHEN YongYan,REN TianHeng

表2 试验中涉及的KASP分子标记引物序列

Table 2 The designed primer sequence of KASP molecular marker in this study

QTL 分子标记 Molecular marker 引物序列 Primer sequence (5′-3′) QSn.sau-2D.2 KASP-AX-109283238-1 GAAGGTGACCAAGTTCATGCTCCATCTATTTTCCCACCATCGTAC KASP-AX-109283238-2 GAAGGTCGGAGTCAACGGATTCCATCTATTTTCCCACCATCGTAT KASP-AX-109283238-3 ACCTTTATCGAGGAAAGAGACACA KASP-AX-111151907-1 GAAGGTGACCAAGTTCATGCTCCTACCCAGCAGCCACAAG KASP-AX-111151907-2 GAAGGTCGGAGTCAACGGATTCCTACCCAGCAGCCACAAT KASP-AX-111151907-3 AAGGCCAGTGTTAGTCCAGC KASP-AX-86163992-1 GAAGGTGACCAAGTTCATGCTGGAAATACTGCTGCTTCTGAACA KASP-AX-86163992-2 GAAGGTCGGAGTCAACGGATTGGAAATACTGCTGCTTCTGAACG KASP-AX-86163992-3 TTGTATGCAGGATGACGCTC KASP-AX-108802182-1 GAAGGTGACCAAGTTCATGCTCATCGAAATGCGGAAATGGAAT KASP-AX-108802182-2 GAAGGTCGGAGTCAACGGATTCATCGAAATGCGGAAATGGAAC KASP-AX-108802182-3 GTGTCTTTGTGTTGATTTGTTTCA KASP-AX-110073027-1 GAAGGTGACCAAGTTCATGCTTGTGAGGACTCAAGAAAACAGC KASP-AX-110073027-2 GAAGGTCGGAGTCAACGGATTTGTGAGGACTCAAGAAAACAGA KASP-AX-110073027-3 ATCCTTTCTGCAGTGGACCC QKl.sau-3D.2 KASP-AX-109962767-1 GAAGGTGACCAAGTTCATGCTTGGAAACAAATTGACTCACATACAA KASP-AX-109962767-2 GAAGGTCGGAGTCAACGGATTTGGAAACAAATTGACTCACATACAG KASP-AX-109962767-3 GTGCTACTCGGCCTCACATA KASP-AX-108923200-1 GAAGGTGACCAAGTTCATGCTGTTGAAGAAACTGCCATCATCAA KASP-AX-108923200-2 GAAGGTCGGAGTCAACGGATTGTTGAAGAAACTGCCATCATCAG KASP-AX-108923200-3 CGTCCAGTGGTTGTCTCAAAC