梅PmARF17克隆及其在花发育中与内源激素的调控模式

李艳林,SHAHID Iqbal,侍婷,宋娟,倪照君,高志红

Isolation of PmARF17 and Its Regulation Pattern of Endogenous Hormones During Flower Development in Prunus mume

LI YanLin,SHAHID Iqbal,SHI Ting,SONG Juan,NI ZhaoJun,GAO ZhiHong

图7 PmARF17 promoter-GUS融合表达载体的结构及PmARF17启动子的瞬时表达 A：pBI121-PmARF17 promoter-GUS融合载体结构图;B：pBI121-PmARF17 promoter-GUS侵染的拟南芥苗GUS染色结果;C：阴性对照GUS染色结果（未经菌液侵染）;D：阳性对照GUS染色结果（pBI121空载侵染）;E：B中根部的放大图;F、G：pBI121-PmARF17 promoter-GUS在花瓣中GUS染色结果;H：pBI121-PmARF17 promoter-GUS在雄蕊中GUS染色结果;I：从H中分离出的蓝色区域高倍镜图;J：阴性对照中花器官GUS染色结果,其雄蕊与H中的雄蕊具有相同组分;K、L：阳性对照中不同根部、花器官、叶片的GUS染色结果

Fig. 7 The structure of PmARF17 promoter-GUS vector and transient expression experiment of PmARF17promoter A: The structure of pBI121-PmARF17 promoter-GUS vector; B: GUS staining results of Arabidopsis thaliana seedlings infected by pBI121-PmARF17 promoter-GUS vector; C: Negative control GUS staining results (without bacterial infection); D: Positive control GUS staining results (infected by bacterial fluid of pBI121 alone); E: Enlarged view of the root in B; F, G: GUS staining results of pBI121-PmARF17 promoter-GUS in petals; H: GUS staining results of pBI121-PmARF17 promoter-GUS in stamens; I: High magnification image of blue area separated from H; J: GUS staining results of floral organ in the negative control, and its stamens have the same components as those in I; K, L: GUS staining results of different roots, flower organs and leaves in the positive control