梅PmARF17克隆及其在花发育中与内源激素的调控模式

李艳林,SHAHID Iqbal,侍婷,宋娟,倪照君,高志红

Isolation of PmARF17 and Its Regulation Pattern of Endogenous Hormones During Flower Development in Prunus mume

LI YanLin,SHAHID Iqbal,SHI Ting,SONG Juan,NI ZhaoJun,GAO ZhiHong

图1 PmARF17的基因结构、系统进化树及PmARF17蛋白与其他植物的同源性比较 A：PmARF17的cDNA和推导的氨基酸序列（下划线标出最大ORF,阴影为保守区）;B：PmARF17蛋白与其他植物的系统进化树分析;C：PmARF17的基因结构;D：PmARF17蛋白与其他植物的同源性比较[蓝色框为B3 DNA binding domain,红色框为Auxin response factor元件。1.Seq：梅;2.Seq：山杏;3.Seq：桃;4.Seq：甜樱桃;5.Seq：苹果;6.Seq：白梨;7.Seq：葡萄;8.Seq：野草莓;9.Seq：杨树;10.Seq：拟南芥;11.Seq：烟草;12.Seq：番茄]

Fig. 1 The gene structure of PmARF17and the phylogenetic tree and homology comparison of PmARF17 protein with other plants A: cDNA and deduced amino acid sequence of PmARF17 (The maximum ORF was underlined and the shaded areas were conservative); B: Phylogenetic tree of PmARF17 protein with other plants; C: The gene structure of PmARF17; D: Homology comparison of PmARF17 protein with other plants [The blue boxes are B3 DNA binding domain, red boxes are Auxin response factor. 1.seq: Prunus mume; 2.seq: Prunus sibirica ; 3.seq: Prunus persica ; 4.seq: Prunus avium ; 5.seq: Malus domestica; 6.seq: Pyrus x bretschneideri ; 7.seq: Vitis vinifera; 8.seq: Fragaria vesca; 9.seq: Populus trichocarpa; 10.seq: A. thaliana; 11.seq: N. tabacum ; 12.seq: Solanum lycopersicum]