利用CRISPR/Cas9技术定向编辑水稻OsIAA11

李兆伟,零东兰,孙聪颖,曾慧玲,刘凯基,蓝颖珊,范凯,林文雄

CRISPR/Cas9 Targeted Editing of OsIAA11 in Rice

LI ZhaoWei,LING DongLan,SUN CongYing,ZENG HuiLing,LIU KaiJi,LAN YingShan,FAN Kai,LIN WenXiong

图4 osiaa11突变体的PCR检测及其与野生型序列比对 A：T2代部分转基因水稻植株OsIAA11编辑靶点附近DNA片段的PCR检测,靶点1的扩增长度为443 bp,靶点2的扩增长度为469 bp,野生型（WT）为ZH11;B：转化植株编辑靶点的PCR产物测序序列与野生型（WT）序列的比对结果,蓝色字母为靶点序列,红色大写字母表示PAM序列,删除线表示缺失碱基,红色小写字母表示插入碱基,-：缺失,+：插入,WT：野生型

Fig. 4 PCR identification and sequence alignment of osiaa11 mutants in comparison with the WT A: PCR amplification results of the DNA fragments near the targets edited of OsIAA11 for partial T2 generation ofosiaa11 mutants, the fragment length of target 1 and 2 is 443 bp and 469 bp, respectively, and WT is ZH11; B: sequence alignment of the edited targets for the osiaa11 mutants and WT line. The blue letters are the target genome sequence; The red capital letters denote PAM; Dashes strikethrough indicate the deleted bases; Insertion nucleotides are shown in red lowercase letters; -: Deletion; +: Insertion; WT: Wild type