利用CRISPR/Cas9技术定向编辑水稻OsIAA11

李兆伟,零东兰,孙聪颖,曾慧玲,刘凯基,蓝颖珊,范凯,林文雄

CRISPR/Cas9 Targeted Editing of OsIAA11 in Rice

LI ZhaoWei,LING DongLan,SUN CongYing,ZENG HuiLing,LIU KaiJi,LAN YingShan,FAN Kai,LIN WenXiong

图1 OsIAA11的gRNA靶位点、氨基酸序列结构及pYLCRISPR/Cas9-IAA11-T12表达载体组装示意图 A：OsIAA11的2个gRNA靶点位置;B：OsIAA11氨基酸序列结构;C：2个靶位点gRNA表达盒与pYLCRISPR/Cas9-MT组装成的表达载体。LB：左边界;RB：右边界

Fig. 1 Target sites of the gRNA in the OsIAA11 gene locus, amino acid sequence structure of OsIAA11, and constructing style of the pYLCRISPR/Cas9-IAA11-T12 vector A: Position of two gRNA targets in the OsIAA11 gene locus; B: Amino acid sequence structure of OsIAA11; C: Cloning of two gRNA cassettes into the pYLCRISPR/Cas9-MT vector. LB: Left border; RB: Right border