利用CRISPR/Cas9技术定向编辑水稻OsIAA11

李兆伟,零东兰,孙聪颖,曾慧玲,刘凯基,蓝颖珊,范凯,林文雄

CRISPR/Cas9 Targeted Editing of OsIAA11 in Rice

LI ZhaoWei,LING DongLan,SUN CongYing,ZENG HuiLing,LIU KaiJi,LAN YingShan,FAN Kai,LIN WenXiong

表2 不同类型osiaa11突变体植株及其野生型水稻（WT）的田间农艺性状

Table 2 Agronomic traits of different type osiaa11 mutants and its corresponding wild type grown in paddy field

株系 Line 株高 Plant height (cm) 有效穗数 Effective panicle number per plant 成穗率 Effective spike rate (%) 穗长 Length of main panicle (cm) 穗粒数 Grain number per panicle 结实率 Seed setting rate (%) 千粒重 1000-grain weight (g) 单株产量 Grain yield per plant (g) 收获指数 Harvest index 谷草比 Grain-straw ratio osiaa11-1-1 69.33±4.03abcd 6.00±0.82b 37.71±4.54b 19.93±0.93bc 128.17±24.12a 87.79±3.72ab 26.22±0.41a 11.21±2.04bcd 0.46±0.031ab 0.95±0.113ab osiaa11-1-2 70.50±2.50abcd 7.00±0.00b 44.44±5.56b 20.35±0.62abc 120.50±9.84a 78.25±16.36ab 25.92±0.78a 12.26±1.24abcd 0.46±0.004ab 0.96±0.000ab osiaa11-8-1 77.50±1.50a 10.50±2.50ab 44.07±4.07b 22.10±1.45abc 130.50±27.98a 86.08±6.46ab 25.98±0.98a 22.45±4.55a 0.44±0.020ab 0.88±0.073ab osiaa11-8-2 74.00±0.82ab 9.67±1.70ab 48.73±4.37b 22.15±1.27abc 128.67±33.21a 88.57±6.58ab 25.68±0.53a 18.69±1.84abc 0.46±0.012ab 0.96±0.021ab osiaa11-11-1 69.00±4.54bc 6.33±1.80b 43.27±7.73b 22.70±1.17a 113.67±23.50a 83.63±6.67ab 24.28±1.11a 10.51±2.67d 0.46±0.038ab 0.98±0.166ab osiaa11-11-2 74.0±0.00abc 4.00±0.00b 26.67±0.00b 21.95±0.70abc 118.25±7.50a 88.65±2.29ab 23.32±0.78a 9.85±2.48d 0.41±0.000ab 0.76±0.000ab osiaa11-17 67.17±1.34bcd 12.00±2.00a 40.53±4.64b 20.47±1.50bc 108.50±13.91a 90.27±8.36a 23.01±1.16a 18.30±3.14a 0.44±0.042ab 0.86±0.149ab osiaa11-19-1 69.30±2.32bc 8.00±1.26b 43.87±3.32b 20.58±0.84abc 118.70±20.27a 83.94±5.15ab 23.21±0.84a 11.72±1.19bcd 0.43±0.051ab 0.84±0.182ab osiaa11-19-2 66.33±1.89bcd 10.33±0.94ab 43.58±2.03b 21.15±0.80abc 109.50±11.38a 87.18±5.55ab 24.43±1.02a 12.20±0.27abcd 0.38±0.033ab 0.67±0.094ab osiaa11-20-1 64.00±1.63cd 5.67±0.47b 50.56±7.49b 21.73±1.84abc 94.17±22.56a 80.43±13.87ab 24.42±1.49a 7.11±2.92d 0.43±0.067ab 0.90±0.269ab osiaa11-20-2 65.50±1.50bcd 6.50±0.50b 54.89±8.74b 22.58±0.74abc 125.50±29.77a 84.28±7.52ab 24.68±0.02a 11.34±2.88bcd 0.52±0.059a 1.28±0.298a osiaa11-21-1 60.50±1.50d 7.00±0.00b 46.88±3.12b 19.82±1.84bc 86.00±4.06a 80.67±15.64ab 21.85±3.35a 6.88±1.62d 0.44±0.017ab 0.85±0.054ab osiaa11-21-2 61.00±0.00cd 4.00±0.00b 57.14±0.00b 18.50±0.40c 78.00±8.00a 64.70±0.41b 23.95±0.0a 3.16±0.00d 0.38±0.000ab 0.72±0.000ab osiaa11-22-1 66.00±1.87cd 8.00±0.71ab 45.75±3.29b 21.09±1.27abc 101.12±14.10a 85.04±5.89ab 23.52±0.94a 11.05±1.67cd 0.44±0.017ab 0.85±0.054ab osiaa11-22-2 65.25±1.09cd 6.50±0.50b 40.29±4.74b 20.50±0.79abc 103.25±19.27a 88.23±6.83ab 23.18±1.24a 9.10±0.43d 0.42±0.027ab 0.79±0.086ab osiaa11-23 64.50±0.50cd 8.00±0.00ab 39.23±2.87b 21.08±0.89abc 124.50±10.62a 84.01±7.71ab 22.40±0.80a 12.29±0.19abcd 0.35±0.037ab 0.59±0.100b osiaa11-25 72.50±0.50abc 11.00±1.00ab 38.52±1.48b 22.92±2.10ab 144.00±47.72a 85.26±7.35ab 24.52±1.08a 20.08±2.34ab 0.35±0.032b 0.58±0.081b WT 69.60±0.80abc 8.00±2.00b 89.78±10.50a 21.16±1.29abc 113.70±16.80a 85.42±6.88ab 22.80±1.53a 10.65±2.75d 0.47±0.036ab 1.08±0.121ab