利用CRISPR/Cas9技术定向编辑水稻OsIAA11
|
|
李兆伟,零东兰,孙聪颖,曾慧玲,刘凯基,蓝颖珊,范凯,林文雄
|
CRISPR/Cas9 Targeted Editing of OsIAA11 in Rice
|
|
LI ZhaoWei,LING DongLan,SUN CongYing,ZENG HuiLing,LIU KaiJi,LAN YingShan,FAN Kai,LIN WenXiong
|
|
表1 所用的引物序列
|
Table 1 The primer sequences used in this study
|
|
| 引物名称Primer name | 引物序列Primer sequence (5′-3′) | 用途Usage | | OsIAA11-T1-fwd | gccgCGCGCGGCTTGTCGGGATC | 靶序列1构建
Construction of target site 1 | | OsIAA11-T1-rev | aaacGATCCCGACAAGCCGCGCG | | OsIAA11-T2-fwd | gttgAGCTACCCGGAGTTGTCCA | 靶序列2构建
Construction of target site 2 | | OsIAA11-T2-rev | aaacTGGACAACTCCGGGTAGCT | | Hyp-F | ACGGTGTCGTCCATCACAGTTTGCC | T0代阳性转基因植株鉴定
Identification of the T0 positive transgenic plant | | Hyp-R | TTCCGGAAGTGCTTGACATTGGGGA | | OsIAA11-T1-F | AGACCATCGACCTCAAGC | 靶点1测序检测
Sequencing for target 1 | | OsIAA11-T1-R | GCTAACTAATGGCTCTTC | | OsIAA11-T2-F | GACGATGATGTCAACTTT | 靶点2测序检测
Sequencing for target 2 | | OsIAA11-T2-R | GATAATGGTAGAATAACTCA | | Hyp-T2-F | CTATTTCTTTGCCCTCGGAC | T2代纯合突变植株体内潮霉素基因检测
Detection of the gene encoded for hygromycin in the T2 homozygous mutations | | Hyp-T2-R | GACGTCTGTCGAGAAGTTTCTG |
|
|
|