基于LC-MS技术研究氟氯苯氰菊酯对西方蜜蜂工蜂幼虫代谢的影响

虞龙涛,杨何妍,苏宇晨,颜伟玉,吴小波

The Effect of Flumethrin on Metabolism of Worker Larvae of Apis mellifera with LC-MS Technique

YU LongTao,YANG HeYan,SU YuChen,YAN WeiYu,WU XiaoBo

表2 对照组与氟氯苯氰菊酯处理组之间差异代谢物

Table 2 Differential metabolites between the control group and flumethrin-treated groups

化合物名称 Compound name 50 mg·kg-1-Control 5 mg·kg-1-Control 0.5 mg·kg-1-Control VIP FC P 趋势 Trend VIP FC P 趋势 Trend VIP FC P 趋势 Trend 尿苷二磷酸-N-乙酰葡糖胺 UDP-N-acetylglucosamine 2.1451 3.7838 0.0175 ↑ 1.0349 2.0512 0.0494 ↑ 1.4664 2.4830 0.0277 ↑ 硫唑嘌呤Azathioprine 3.5178 22.9131 0.0038 ↑ 2.2240 6.6142 0.0216 ↑ 2.8184 12.1241 0.0135 ↑ 反玉米素Trans-zeatin 3.4573 27.3706 0.0012 ↑ 2.1436 10.783 0.0109 ↑ 3.5919 29.5094 0 ↑ 焦炭酸二乙酯Diethylpyrocarbonate 1.8239 2.6369 0.0126 ↑ 1.3799 2.1616 0.0312 ↑ 1.8167 2.6558 0.0272 ↑ 阿斯巴甜 Methyl alpha-aspartylphenylalaninate 2.4076 6.6251 0.0210 ↑ 1.5393 3.6759 0.0056 ↑ 2.5201 7.6114 0.0272 ↑ 草完隆Noruron 2.5040 5.7411 0.0237 ↑ 1.8949 5.3422 0.0312 ↑ 2.4082 5.7124 0.0272 ↑ 四聚乙醛Ethanal tetramer 3.1358 10.8657 0.0022 ↑ 1.6834 4.3428 0.0168 ↑ 1.9323 4.0922 0.0240 ↑ 扎莫特罗Xamoterol 1.7021 2.5619 0.0303 ↑ 1.6402 2.9414 0.0271 ↑ 2.3289 4.8191 0.0272 ↑ 二甲弗林Dimetofrine 2.0830 5.3298 0.0337 ↑ 1.9187 6.1752 0.0216 ↑ 2.3776 6.4440 0.0434 ↑ 细辛脑Asarone 3.5178 22.9131 0.0038 ↑ 2.2168 7.7327 0.0301 ↑ 2.4104 5.5813 0.0399 ↑ 异丙酚Propofol 1.9412 3.3387 0.0169 ↑ 1.7439 3.4319 0.0121 ↑ 2.2298 4.1785 0.0135 ↑ 芹黄素Apigetrin 2.7393 7.0597 0 ↑ 2.1954 5.8356 0.008 ↑ 2.2192 5.1848 0.0070 ↑ 4,4'-二硝基联苄4,4'-dinitrobibenzyl 3.5924 36.459 0.0024 ↑ 1.7819 5.2543 0.0073 ↑ 3.1705 15.6981 0.0019 ↑ 早熟素Precocene ii 2.8581 11.2521 0.0012 ↑ 1.0312 2.0035 0.0439 ↑ 2.1354 3.8515 0.0428 ↑ 贝那普利拉Benazeprilat 1.3429 2.0242 0.0438 ↑ 1.8099 6.4223 0.0188 ↑ 2.6353 10.2252 0.0135 ↑ 氟扎可特Azacortid 2.6960 12.7132 0.0305 ↑ 2.4081 15.5769 0.0188 ↑ 3.5251 35.6758 0.0048 ↑ 甲苯Toluene 1.5881 0.4467 0.0226 ↓ 1.4841 0.2876 0.0301 ↓ 1.9802 0.2721 0.0235 ↓ 愈伤酸Traumatic acid 2.4552 0.1909 0.0021 ↓ 1.5634 0.2683 0.0121 ↓ 2.0103 0.2575 0.0079 ↓ 9-羟基壬酸9-oxononanoic acid 2.9634 0.0980 0.0006 ↓ 1.3122 0.2638 0.0139 ↓ 2.7705 0.1129 0.0015 ↓ 普瑞巴林Pregabalin 1.6876 0.4322 0.0028 ↓ 1.2720 0.4581 0.0178 ↓ 1.0768 0.5848 0.0389 ↓ 阿伐那非Avanafil 1.9936 0.3237 0.0253 ↓ 1.1258 0.4234 0.0347 ↓ 1.7236 0.3553 0.0235 ↓ 1-壬酸1-nonanoic acid 2.6990 0.1060 0.0104 ↓ 2.0052 0.1232 0.0238 ↓ 2.5335 0.1151 0.0260 ↓ 匹伐加宾Pivagabine 2.8956 0.1266 0.0057 ↓ 2.0864 0.1468 0.0560 ↓ 2.1990 0.1935 0.0135 ↓ 美噻吨Metixene 2.6297 0.1776 0.0012 ↓ 1.1710 0.3815 0.0271 ↓ 1.4653 0.3801 0.0277 ↓ 樟脑(+/-)-camphor 2.2568 0.2106 0.0169 ↓ 1.4478 0.2932 0.0247 ↓ 2.2074 0.2177 0.0216 ↓ 扎莫特罗Xamoterol [usan:ban:inn] 1.8083 0.2538 0.0229 ↓ 2.1189 0.1402 0.0056 ↓ 1.8550 0.2617 0.0235 ↓ 二甲福林Dimetofrine 2.5825 0.1265 0.0139 ↓ 2.1321 0.1535 0.0238 ↓ 2.6898 0.1118 0.0199 ↓ 苯乙酮Acetophenone 1.7796 0.3617 0.0213 ↓ 1.8288 0.2989 0.0139 ↓ 1.5460 0.4091 0.0387 ↓ 氢过氧化亚油酸13(s)-HPODE 1.1855 0.5253 0.0414 ↓ 1.6963 4.7824 0.0331 ↑ 1.5777 0.4183 0.0235 ↓