14-3-3蛋程中的互作靶蛋白鉴定及其对外源激素的响应

张志兴,敏秀梅,宋果,陈花,许海龙,林文雄

Identification of 14-3-3 Client Proteins in Rice Grains and Their Response to Exogenous Hormones During the Grain Filling Stage

ZHANG ZhiXing,MIN XiuMei,SONG Guo,CHEN Hua,XU HaiLong,LIN WenXiong

图2 水稻籽粒中GF14b和GF14e靶蛋白的SDS-PAGE检测分析 A：F14b蛋白亲和层析结果的SDS-PAGE分析;B：GF14e蛋白的亲和层析结果的SDS-PAGE分析;条带1为空白对照;条带2为阴性对照; 图A中条带3和4为GF14b的亲和层析的洗脱蛋白液;图B中条带3和4为GF14e的亲和层析的洗脱蛋白液;图A中的条带5为纯化的GF14b蛋白;图B中的条带5为纯化的GF14e蛋白;条带6为籽粒非变性蛋白;条带7为marker

Fig. 2 SDS-PAGE analysis of GF14b and GF14e client proteins in rice grain A:SDS-PAGE analysis of GF14b client proteins; B: SDS-PAGE analysis of GF14e client proteins; Line 1 is the blank control; Line 2 is the negative control; Line 3 and line 4 in Fig. A are the elution protein of affinity chromatography of GF14b; Line 3 and line 4 in Fig. B are the elution protein of affinity chromatography of GF14e; Line 5 in Fig. A is the purified GST-GF14b protein; Line 5 in Fig. B is the purified GST-GF14e protein; Line 6 is the crude protein from developing rice grains; Line 7 is the marker