14-3-3蛋程中的互作靶蛋白鉴定及其对外源激素的响应

张志兴,敏秀梅,宋果,陈花,许海龙,林文雄

Identification of 14-3-3 Client Proteins in Rice Grains and Their Response to Exogenous Hormones During the Grain Filling Stage

ZHANG ZhiXing,MIN XiuMei,SONG Guo,CHEN Hua,XU HaiLong,LIN WenXiong

表1 试验中用到的qRT-PCR和PCR的引物

Table 1 qRT-PCR and PCR primers used in this study

基因 Gene Accession No. 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) Primer for qRT-PCR GF14a LOC_Os08g37490 AGCCATGAAGGAGCTGTCGC GCTCATCCTCAGGCTTGGTT GF14b LOC_Os04g38870 GCTTGAATCCCACCTTGTC AATGTCCTGAGCAGCCTTG GF14c LOC_Os08g33370 CGTTTGACGAAGCCATCTCC CTAGTAGAACAGGAGAAGAATC GF14d LOC_Os11g34450 TGCTCTCGCAGATTTGGCTC ATCCCCAGGCTCTTTTGGAG GF14e LOC_Os02g36974 GATATTGCCCTGGCAGAGTTG GAGATATCGGAAGTCCACAGC GF14f LOC_Os03g50290 AGCAGCTGAGAACACTCTTG CAGCAATAGCATCGTCGAAC GF14g LOC_Os01g11110 AGCGACGACCTCGTCTACAT TGACTCTCCTTGCCCTTTGT GF14h LOC_Os11g39540 TTATGGCCTATCAGGCTTGG TTCTCCTTCAGGAGCTGCAT AGPS LOC_Os08g25734 TTACTGGGAAGACATTGGTACC CTCCCATGAGTAATGAGTCCTC AGPL LOC_Os01g44220 GGAAAGATTGAATATTGGGGGC TCAGAGGAAAGAGTTGAACTCC SBE LOC_Os02g32660 GATCAGTATGAAGGAGGACTGG ACCTACTAATGCTGCAGAATGT SUS2 LOC_Os06g09450 GGAGAAAACCAAATACCCCAAC CAGTGTGATTCATGGCGATAAG PPDK2 LOC_Os03g31750 CTAGCGGAATTCTTCTCGTTTG CAAATGCCCACCTCTAAATCAG β-actin LOC_4333919 CTGCGGGTATCCATGAGACT GCAATGCCAGGGAACATAGT Primer for PCR GF14b LOC_Os04g38870 TTTTTTGGATTCATGTCGGCACAGGCGGAGCTTTCC TATATATACTCGAGCTGCCCCTCGCTGGAGTCGCGCTT GF14e LOC_Os02g36974 TTTCGAATTCATGTCGCAGCCTGCTGAGCTTTCCC TTTCTCGAGCTGTCCATCTCCTGATTCGCCCTTGT SUS3 LOC_Os07g42490 TCCGAATTCATGGGGGAAACTACTGGAGAACGTGC TTTTCTCGAGTTTGGTGGAGGCCTCTCCCTCAATG PSA LOC_Os02g12650 GGGGGGGAATTCATGCATGGCTTCTACAGAAGTGTG GAGCTCGAGGTGGTCGTGAGAAATTTCCTTGAGGAC