48份玉米自交系抗病性的精准鉴定

赵子麒,赵雅琪,林昌朋,赵永泽,余宇潇,孟庆立,曾广莹,薛吉全,杨琴

Precise Evaluation of 48 Maize Inbred Lines to Major Diseases

ZHAO ZiQi,ZHAO YaQi,LIN ChangPeng,ZHAO YongZe,YU YuXiao,MENG QingLi,ZENG GuangYing,XUE JiQuan,YANG Qin

表2 已克隆抗病基因鉴定引物

Table 2 Primers used to identify the cloned disease resistance genes

基因名称 Gene ID 病害 Disease 引物名称 Primer name 引物序列 Primer sequence (5′-3′) qRfg1 禾谷镰孢茎腐病 Gibberella stalk rot TED-F GCACAAGAGAGATGGAGCATT TED-R ATTCTCAATCCAAGGTGCAG TERB-F CCTAAGAACCGTCGGAAACA TERB-R CGAGCGTTTTCGACATAACA TELB-F AAACGCTGACACTTCCGACT TELB-R GTCGACACGTGTAGGAAGCA Rcg1 炭疽茎腐病 Anthracnose stalk rot FLP-111-F TTCCTGTTCGTCTGTATCTGATCCG FLP-111-R TTTGATTCCGGTCGAGTATAACCTG FLP-112-F GAAACTGCCTTCCCAGAAAACAATG FLP-112-R CAAGATCGGTGAAGTTGGTGCTTC FLP-113-F ATCACAGATGGGTCTCAAGGATTGC FLP-A1-R TTCCAAGCAATTCACAGCTC Htn1 大斑病 Northern leaf blight Htn1-F TCTTCTCCCCGCCATGGC Htn1-R CGGTGTAGCCGCAGGAGT ZmCCoAOMT2 小斑病、灰斑病 Southern leaf blight, Gray leaf spot CAMT-F CCGTCCTGGACGACCTCGTG CAMT-R TGCTGCGCGTCGTCTACGAT