水稻粒宽突变体gw87的基因定位及候选基因分析

张翔宇,郭佳,王三,陈聪萍,孙昌辉,邓晓建,王平荣

Gene Mapping and Candidate Gene Analysis of Grain Width Mutant gw87 in Rice

ZHANG XiangYu,GUO Jia,WANG San,CHEN CongPing,SUN ChangHui,DENG XiaoJian,WANG PingRong

图6 BR生物合成途径基因在突变体gw87和野生型676R（WT）中的表达分析 A：苗期突变体和野生型中OsDWARF4、D11和D2的表达水平。在每个基因在野生型中表达量设为1.0,以此计算出突变体基因的表达量。B：用BL处理突变体和野生型幼苗后OsDWARF4、D11和D2的表达水平。幼苗在黑暗中水培10 d,剪取第1叶叶环连接部位约0.5 cm叶片和叶鞘,用100 nmol·L-1 BL处理3 h,提取RNA进行检测

Fig. 6 Expression analysis of the genes for BR biosynthesis in gw87 mutant and its wild type (WT) A: The relative transcript levels of OsDWARF4, D11 and D2 in gw87 and its wild type at seedling stage. The transcript level of each gene in wild type was defined as 1, and that in gw87 mutant was calculated accordingly. B: The relative transcript levels of OsDWARF4, D11 and D2 in gw87 and its wild type without or with 100 nmol·L-1 BL treatment for 3 h. Total RNAs were extracted from 0.5 cm of the lamina joints of the first intact leaf