水稻粒宽突变体gw87的基因定位及候选基因分析

张翔宇,郭佳,王三,陈聪萍,孙昌辉,邓晓建,王平荣

Gene Mapping and Candidate Gene Analysis of Grain Width Mutant gw87 in Rice

ZHANG XiangYu,GUO Jia,WANG San,CHEN CongPing,SUN ChangHui,DENG XiaoJian,WANG PingRong

图5 gw87突变体的基因定位及候选基因分析 A：突变基因定位于SSR标记RM163和InDel标记X1之间;B：突变基因定位于InDel标记X2和X3之间101 kb染色体区域;C：该101 kb区域含有15个注释基因;D：突变体中候选基因LOC_Os05g32270的第1 041位碱基G突变为A,导致该位点相邻的76 bp内含子序列被剪接为外显子,外显子和内含子序列分别用大写和小写字母表示,箭头表示突变位点;E：突变体LOC_Os05g32270的cDNA中含76 bp内含子特异序列的扩增;M：DL5000 DNA Marker

Fig. 5 Gene mapping and candidate gene analysis of gw87 mutant A: The gw87 locus was mapped to a region between SSR marker RM163 and InDel marker X1 on chromosome 5; B: The locus was narrowed down to a 101-kb genomic region between InDel markers X2 and X3; C: The 101 kb region contains 15 putative genes; D: A single nucleotide G-to-A substitution occurred at position 1041 in candidate gene LOC_Os05g32270 in the gw87 mutant, which was indicated with an arrow. Due to the point mutation, 76 bp of intron sequences were spliced into exons in the cDNA sequence of LOC_Os05g32270 in the mutant. The sequences of extron and intron are shown in upper and lower case, respectively; E: Amplification of LOC_Os05g3227 cDNA containing the specific sequence of 76 bp intron in gw87. M: DL5000 DNA Marker