水稻粒宽突变体gw87的基因定位及候选基因分析

张翔宇,郭佳,王三,陈聪萍,孙昌辉,邓晓建,王平荣

Gene Mapping and Candidate Gene Analysis of Grain Width Mutant gw87 in Rice

ZHANG XiangYu,GUO Jia,WANG San,CHEN CongPing,SUN ChangHui,DENG XiaoJian,WANG PingRong

表6 gw87突变基因的MutMap分析结果以及用于精细定位的InDel和SSR标记

Table 6 MutMap results of the gw87 gene, and InDel and SSR makers developed for fine mapping of gw87

标记名称 SNP and marker name 物理位置 Physical location (bp) SNP指数 SNP Index 注释基因及碱基突变位点,或分子标记引物序列 Annotated gene and its nucleotide mutation site, or marker primers SNP01 9834287 0.956 LOC_Os05g17170 downstream, G2464A SNP02 10490240 0.920 LOC_Os05g18240 downstream, G1713A SNP03 11888797 0.909 LOC_Os05g20290 upstream, -2662G>A SNP04 12878107 1 LOC_Os05g22680 downstream,79G>A SNP05 13399064 0.962 Intergenic_region, LOC_Os05g23430-LOC_Os05g23440,13399064C>T SNP06 13926842 0.960 LOC_Os05g24140 upstream,-1434C>T SNP07 13966225 0.958 LOC_Os05g24190 upstream,-3398C>T SNP08 14365938 0.906 LOC_Os05g24790 upstream,-2425C>T SNP09 14647328 0.913 LOC_Os05g25240,synonymous,93C>T|p.Cys31Cys SNP10 14908086 0.960 LOC_Os05g25640 upstream_gene, -3616G>A X1 18307365 F: TATAGCCAGGATGAGAAG, R: CTGCTGTGGATCATCTTG X2 18753900 F: GTACCCATCATAGCAAGA, R: AATAGGGTTGGAAGAATG SNP11 18813290 1 LOC_Os05g32270, splice_donor_variant&intron_variant,1040+1G>A X3 18854840 F: CTATTTGCTATCGGACAC, R: ATTACTCTTTGGGTTTGG SNP12 18932427 1 LOC_Os05g32430 downstream,117G>A X4 18932825 F: CGGCAACAAGAGGAGGAT, R: ACAGCAATGTCCGCAACGGTTTACA RM163 19251955 F: ATCCATGTGCGCCTTTATGAGGA, R: CGCTACCTCCTTCACTTACTAGT