基于适配体的光学和电化学法对食源性致病菌检测的研究进展

惠媛媛,彭海帅,王毕妮,张富新,刘玉芳,贾蓉,任荣

Research Progress of Food-Borne Pathogen Detection Based on Electrochemical and Optical Aptasensors

HUI YuanYuan,PENG HaiShuai,WANG BiNi,ZHANG FuXin,LIU YuFang,JIA Rong,REN Rong

表3 用于食源性致病菌检测的传感器性能比较

Table 3 Comparison of analytical properties of the aptasensors for food-borne pathogen detection

传感器类型 Sensor type 方法 Method 策略 Strategy 检测范围 Detection range (cfu·mL-1) 检测限 LOD (cfu·mL-1) 参考文献 Reference 电化学 Electrochemical 将适配体固定在电极表面,靶标与适配体的结合导致电极表面阻抗增大,通过检测电化学信号的变化检测靶标 The aptamer is modified on the surface of the electrode, and the combination of the target and the aptamer leads to an increase in the surface impedance of the electrode. The target is detected by detecting the change of electrochemical signal rGO-TiO2复合纳米材料信号放大 RGO -TiO2 composite nanomaterial signal amplification 101—108 10 [62] rGO-CNT复合纳米材料信号放大 rGO-CNT composite nanomaterial signal amplification 101—108 10 [63] 聚[吡咯-co-3-羧基吡咯]共聚物信号放大 poly[pyrrole-co-3-carboxyl-pyrrole]copolymer signal amplification 102—108 3 [64] 三明治法 Sandwich assay 适配体-靶标-MB@MI纳米材料 Apt-target-MB@MI 102—108 32 [65] 抗体/磁性纳米颗粒-靶标-适配/脲酶复合物 Antibody/magnetic nanoparticles - target - aptamer/urease complex 101—104 10 [66] 三明治法 Sandwich assay 滚环扩增 Rolling cycle amplification 2.2—2.2×108 2 [67] 比色 Colorimetric NaCl-AuNPs 高浓度的盐会引起AuNPs聚集,颜色从酒红色变为蓝色 high-salt induce AuNPs aggregation from red to blue _ 105 [68] 102—107 56 [69] 105—108 7.2×105 [70] 过氧化氢氧化法 H2O2 oxidation 7-四链体-heminDNA酶可催化四甲基联苯胺（TMB）-过氧化氢（H2O2）体系发生反应,颜色从无色变为蓝色 G4/Hemin DNAzyme can catalyze TMB-H2O2 reaction system, and colour of the substrates changing from colourless to blue 102—107 10 [71] 纳米模拟酶可催化TMB-H2O2 体系发生反应,颜色从无色变为蓝色 Nanozymes can catalyze TMB-H2O2 reaction system, and colour of the substrates changing from colourless to blue 11—1.1×105 11 [72] 102—106 10 [73] 荧光 Fluorescent 荧光的产生 Fluorescence generation TPE-OH 能在牛血清白蛋白（BSA）微球中聚集,产生明亮的荧光信号 TPE-OH aggregates in bovine serum albumin (BSA) microspheres producing bright fluorescent signal 101—106 10 [74] 荧光的猝灭 Fluorescence quenching MoS2纳米材料对于荧光素（FAM）的猝灭 Quenching of MoS2 nanomaterials to fluorescein (FAM) 102—105 10 [75] CIP@MWCNT纳米材料对于荧光素（FAM）的猝灭 Quenching of CIP@MWCNT nanomaterials to fluorescein (FAM) 7.15—103 3.15×102 [76] 荧光染料AccuBlue? Fluorescent dye AccuBlue? 靶标与适配体的结合会改变荧光染料的荧光信号 The binding of target and aptamer changed the fluorescence signal of fluorescent dye 50—106 25 [77] 荧光染料罗丹明B Fluorescent dye Rhodamine B 1530—96938 464 [78] 化学发光 Chemiluminescence 化学发光共振能量转移 Chemiluminescence resonance energy transfer Co2+增强/ABEI功能化花状纳米金和滚环扩增技术 Co2+ enhanced /ABEI functionalized flower-like nanogold and roll ring amplification techniques 32—3.2 ×106 10 [79] 50—1.5×105 15 [80] 表面增强拉曼散射 SERS 将适配体修饰在SERS基地上,当靶标与适配体结合后会产生拉曼信号变化,通过对拉曼变化信号的检测进而检测靶标菌 The aptamer is modified on the SERS base. When the target and the aptamer are combined, the Raman signal will change, and the target bacteria can be detected by detecting the Raman change signal 适配体和罗丹明 B 同时修饰的金纳米棒作为拉曼信号探针 The gold nanorods modified by aptamer and rhodamine B was used as Raman signal probe 101—104 3 [81] 适配体和拉曼分子修饰的金纳米颗粒作为拉曼信号探针 The nanoparticles modified by aptamer and raman molecules was used as Raman signal probe 102—107 35/15 [82] 适配体和罗丹明修饰的Au@Ag 核壳纳米材料作为拉曼信号探针 The Au@Ag core-shell nanomaterials modified by ROX and aptamer was used as Raman signal probe 15—1.5×105 15 [83] 表面等离子体共振 Surface plasmon resonance 将适配体修饰在等离子共振装置表面上。当靶标菌存在时共振装置折射率、反射光等会发生变化,通过对光变化信号的检测可以检测靶标 The aptamer is modified on the surface of the plasmon resonance device. When the target bacteria are present, the refractive index and reflected light of the resonance device will change, and the target can be detected by detecting the light change signal 使用Ω形光纤探针 Using a novel Ω-shaped ?ber-optic probe 5.0×102— 1.0×108 5 [84]