基于适配体的光学和电化学法对食源性致病菌检测的研究进展

惠媛媛,彭海帅,王毕妮,张富新,刘玉芳,贾蓉,任荣

Research Progress of Food-Borne Pathogen Detection Based on Electrochemical and Optical Aptasensors

HUI YuanYuan,PENG HaiShuai,WANG BiNi,ZHANG FuXin,LIU YuFang,JIA Rong,REN Rong

表2 食源性致病菌的适配体序列

Table 2 Aptamers of food-borne pathogens

食源性致病菌 Pathogen 菌型 Bacterial type 筛选方法 Screening method 靶标 Target 适配体序列 Sequence (5′-3′) 参考文献 Reference 大肠杆菌 Escherichia coli O157:H7 Non-SELEX 脂多糖 LPS TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC [42] SELEX 全菌 Whole bacteria ACCAGTAGACTTTCAACTTTACTGCCATCGTGTGCCCTAA [43] Non-SELEX 脂多糖 LPS CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG [44] EPEC SELEX 全菌 Whole bacteria ACAGTGCTCGGGATATATCAATATGTCACCTCGGCTAATG [45] O55:B5 Non-SELEX 脂多糖 LPS TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA [46] 沙门氏菌 Salmonella 鼠伤寒 Typhimurium Whole-cell-SELEX 全菌 Whole bacteria ACGGGCGTGGGGGCAATGCCTGCTTGTAGGCTTCCCCTGTGCGCG [47] SELEX 全菌 Whole bacteria TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG [48] SELEX IVB型菌毛 IVB pili GGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUAGGAUCCCGC [49] O8 Whole-cell-SELEX 全菌 Whole bacteria GATCCGGGCCTCATGTCGAACACCCCCCAACTAAAACAACAAAACACCACCGCCATTGAGCGTTTATTCTGAGCTCCCA [50] SELEX 全菌 Whole bacteria TGATCCGGGCCTCATGTCGAACCCACACCCCACAACCACCCAGCCCCAGCCCGCTATTGAGCGTTTATTCTGAGCTCCCA [51] 金黄色葡萄球菌 Staphylococcus aureus SELEX 磷壁酸 Teichoic acid GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU [52] SELEX 肠毒素C1 Enterotoxin C1 AGCAGCACAGAGGTCAGATGTATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGTCCTATGCGTGCTACCGTGAA [53] SELEX 肠毒素B Enterotoxin B AGCAGCACAGAGGTCAGATGTATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGTCCTATGCGTGCTACCGTGAA [54] SELEX 肠毒素B Enterotoxin B TGCAGGATCCGGTATCCGTGGACGGTGTGCAGGATCCGGTATCCGTGGGCACGAGAATTCCTCCGTTGCG [55] SELEX 肠毒素A Enterotoxin A TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC [56] 单核增生李斯特菌 Listeria monocytogenes Whole-cell-SELEX 全菌 Whole bacteria TGGGAGCTCAGAATAAACGCTCAACTTTGTTCTTCTTTGCTTTTTTTTTCTTTTTTTGTTCGACATGAGGCCCGGATCA [57] 阪崎肠杆菌 Cronobacter sakazakii Whole-cell-SELEX 全菌 Whole bacteria ATAGGAGTCACGACGACCAGGCCGCCCGGGGACGGGGGCGGCGGGGAGGAGGGCGGCGGGTATGTGCGTCTACCTCTTGA [58] 蜡样芽孢杆菌 Bacillus cereus SELEX 芽孢 Spore CATCCGTCACACCTGCTCGGTGCAGACCCATAGGGGGGGCGTGCGGATGTAGGAGTAGGGTGTTGGCTCCCGTATC [59] 铜绿假单胞菌 Pseudomonas aeruginosa Whole-cell-SELEX 全菌 Whole bacteria CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGC TTCCTTTCTTG [60] 副溶血性弧菌 Vibrio parahemolyticus Whole-cell-SELEX 全菌 Whole bacteria ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT [61]