谷子抽穗时间基因SiTOC1的表达与单倍型变异分析
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张林林,智慧,汤沙,张仁梁,张伟,贾冠清,刁现民
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Characterizations of Transcriptional and Haplotypic Variations of SiTOC1 in Foxtail Millet
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ZHANG LinLin,ZHI Hui,TANG Sha,ZHANG RenLiang,ZHANG Wei,JIA GuanQing,DIAO XianMin
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表2 SiTOC1 PCR扩增和测序引物
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Table 2 Primers for amplication and sequencing of SiTOC1
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| 产物Product | 引物名称 Primer name | 引物序列 Primer sequence (5′-3′) | 产物长度 Product length (bp) | 编码区
Coding region | G5-F1 | GATCCAGCGACAGTCCA | 1450 | | G5-R1 | AGACAGGTCGGACTGAAATA | | G5-F2 | GATGAGTTGCCACAAAGATG | 1272 | | G5-R2 | TGTATTGCCTTTCCCAGTAG | 启动子区
Promoter region | G5P-F1 | GGCCAAACGAAACCATG | 2069 | | G5P-R1 | CCTAATCCGGGAAACCAG | | G5P-F1.1 | ATTGCGTGCACGAATCT | | | G5P-R1.1 | CAGCAGCAGCCTGCCTCG | SiTOC1互补DNA
SiTOC1 cDNA | G5Q-F1 | GCCGATCAAGCATCATATGTTAAGT | 250 | | G5Q-R1 | TTTGGCCTTCATTGCTTCGC | Cullin互补DNA
Cullin cDNA | Cullin-F | TATGGGTCATCAACAGCTTGTC | 112 | | Cullin-R | GTAGTCCCTCGTGATGAGATCC |
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