谷子抽穗时间基因SiTOC1的表达与单倍型变异分析

张林林,智慧,汤沙,张仁梁,张伟,贾冠清,刁现民

Characterizations of Transcriptional and Haplotypic Variations of SiTOC1 in Foxtail Millet

ZHANG LinLin,ZHI Hui,TANG Sha,ZHANG RenLiang,ZHANG Wei,JIA GuanQing,DIAO XianMin

表1 SiTOC1亚细胞定位载体构建PCR扩增及测序引物

Table 1 PCR amplification and sequencing primers for SiTOC1 subcellular localization vector’s construction

载体 Vector 引物名称 Primer name 引物序列 Primer sequence (5′-3′) 产物长度 Product length (bp) GFP-N 端载体 GFP-N terminal vector GFPG5-F1 GCTGTACAAGACTAGTATGGTGGGCGGCGGCG 1517 GFPG5-R1 GGGGAAATTCGAGCTCCTACTCTGGAGAAGAAATAATC PUCGFPN-TestF TACAACTACAACAGCCACAA 2163 PUCGFPN-TestR CCTCTTCGCTATTACGC GFPG5-F1.1 CCATCATCATGATGTCC GFPG5-R1.1 TTCAGAGCGACTGCATG GFP-C端载体 GFP-C terminal vector G5GFP-F1 TAGTGGATCCATCGATATGGTGGGCGGCGGCG 1514 G5GFP-R1 TCCCGGGAGCGGTACCCTCTGGAGAAGAAATAATCTC PUCGFPC-TestF ACCTCCTCGGATTCCAT 2354 PUCGFPC-TestR TGCCGTTCTTCTGCTTG G5GFP-F1.1 CAAGATGCTCAAGTACA G5GFP-R1.1 CGATTTACATACCTCACT RFP-N端载体 RFP-N terminal vector MYBRFP-F GCTGTACAAGACTAGTATGGACATGGCGCACGAGAG 1022 MYBRFP-R GGGGAAATTCGAGCTCTCACACGGCGGCCTGGGT PUCRFPN-TestF CTCAAGCTCAAGGACGG 1584 PUCRFPN-TestR CCTCTTCGCTATTACGC