甜瓜种皮颜色控制基因CmSC1的精细定位及候选基因分析

马建,李丛丛,黄亚婷,谢雨黎,程玲玲,王建设

Fine Mapping and Candidate Gene Analysis of Seed Coat Color Gene CmSC1 in Melon

MA Jian,LI CongCong,HUANG YaTing,XIE YuLi,CHENG LingLing,WANG JianShe

表1 本研究所用的PCR引物序列

Table 1 PCR primer sequences used in this study

引物名称 Primer 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') ID1716 TTCCACGAACTCAGGAGCTG AGTAGCATGAGGCTAGACTTGA S24 TGAACTCGTGTCTAACGTACCA ACTCCACTCTCGTATCCAGT S26 GCAATGGAGGTGAGTGCCAA GCATGTCTTTGCCATGTTGTGT S27 ATGACCAAACAATGGTGCTGTG GCTTGTGTGAGAGTAAATCAAGGT S2 AGAGGGAAGCCATCAAGCAA ATTGTACATACTGTCTAGGGTTTCT S28 AAACCATTGACACAAGCTCCA ACATCACATGTATCAAGTGCCT S29 ATTCCATTTTCGTCAAACAACTTTC GTCCATCATGATCTATCGCAAAA S30 GGCTTTCCTTTGTCAGATTCCA CCAAAGTGGGGTAGGAGCATT S33 GGTTTATGATGAAAACGACCGA GAGGTGACGAGGTCCAAATAA S34 CACCCACTTAGGGTTGAAGAA GTTTGGAATTGAAGTACACACCT S35 GAGCATTAAGACCAAAGACACAA TAAGTAACGAGTGAGGTTGGG ID3716 GCTACAAGCCATGTTGAACTCT TGAAGAAGCGAGGAAAGAATAGG YS CTTATCCGCCTCCGACACC AGTTAACGCACCCGAACCC CmSC1 AGACCCATTTGTTTCACTTTCACC CAGAGAAGCTTCCACTCCCA