病毒诱导基因沉默在蔬菜作物上应用的研究进展

李杰,罗江宏,杨萍

Research Advances of Applying Virus-Induced Gene Silencing in Vegetables

LI Jie,LUO JiangHong,YANG Ping

表2 利用VIGS研究的瓜类、叶菜类和豆类蔬菜基因及其表型

Table 2 List of genes silenced by VIGS and the phenotype observed in melon, leaf and legume vegetable

物种 Species 靶基因 Target gene 载体 Vector 功能 Function 沉默表型 Silencing phenotype 参考文献 Reference 黄瓜 Cucumber GPAT6 TRV 不饱和脂肪酸的合成 Unsaturated fatty acids synthesis 抗性增强 Enhanced resistance [62] 甜瓜 Muskmelon LOX10 TRV 脂氧合酶合成 Lipoxygenase synthesis 细胞程序性死亡 Programmed cell death [63] 菠菜 Spinach GAL BCTV 调控开花 Regulation flowering 雌花结构改变 Changed female flower [64] 叶用莴苣 Leaf lettuce Hsp70 TRV 耐热性 Thermotolerance 茎伸长 Stem elongation [65] 白菜 Chinese Cabbage BrAN3, BrBRM TYMV 细胞伸长 Cell elongation 叶片卷曲,小叶增多 Leaf curled and increased [66] 豌豆 Pea CHLI PEBV 叶绿素的合成 Chlorophyll synthesis 叶片发黄 Leaf yellowing [67] 豌豆 Pea PsPIP2 PEBV 水通道蛋白合成 Aquaporin synthesis 叶片和根系衰退 Organ declines of leaf and root [68]