病毒诱导基因沉默在蔬菜作物上应用的研究进展

李杰,罗江宏,杨萍

Research Advances of Applying Virus-Induced Gene Silencing in Vegetables

LI Jie,LUO JiangHong,YANG Ping

表1 利用VIGS研究的茄果类蔬菜基因及其表型

Table 1 List of genes silenced by VIGS and the phenotype observed in solanaceous vegetable

物种 Species 靶基因 Target gene 载体 Vector 功能 Function 沉默表型 Silencing phenotype 参考文献 Reference 番茄 Tomato KAS III, KAS IV/II-like TRV 酰基糖代谢 Acyl glucose metabolism 直链脂肪酸减少 Reduced straight-chain fatty acids [36] 番茄 Tomato SlSWEET1 TRV 糖的转运 Sugar transport 己糖含量降低 Decreased hexose [37] 辣椒 Pepper CaMYB108 TRV 辣椒素合成 Capsaicin synthesis 花粉延迟开裂 Delayed pollen cracking [38] 辣椒 Pepper pAMT ALSV 辣椒素合成 Capsaicin synthesis 辣椒素含量降低 Capsaicin content decreased [39] 番茄 Tomato SLAR TRV 类胡萝卜素合成 Carotenoids synthesis 类胡萝卜素含量降低 Carotenoids content decreased [40] 茄子 Eggplant PDS, CHLI, CHLH TRV 镁螯合酶 Magnesium chelatase 叶片发黄 Foliage yellowing [41] 茄子 Eggplant CHS TRV 类黄酮的合成 Flavonoids synthesis 果实颜色变浅,果实弯曲 Changed fruit color and fruit curved [42] 番茄 Tomato SlILL TRV 生长素合成 Auxin synthesis 加速离层 Accelerate the abscission layer [43] 番茄 Tomato LeCTR1 TRV 乙烯合成 Ethylene synthesis 叶片弯曲减轻 Reduced leaf curve [44] 番茄 Tomato DEK TRV 抗病性 Disease resistance 抗病性减弱 Attenuates disease resistance [45] 茄子 SPDS TRV 抗枯萎病 Resistant to wilt 抗病性减弱 Attenuates disease resistance [46] 辣椒 Pepper CaPHL8 TRV 青枯病 Bacterial wilt 抗病性减弱 Attenuates disease resistance [47] 番茄 Tomato SAHH1, MS1, GAD2 TRV 青枯病 Bacterial wilt 抗病性减弱 Attenuates disease resistance [48] 番茄 Tomato ShORR-1 TRV 白粉病 Powdery mildew 叶片病斑增多 Leaf lesions increased [49] 辣椒 Pepper CaWRKY45, CaWRKY58 TRV 抗病和抗旱 Resistance to disease and drought 抗性减弱 Attenuates disease resistance [50] 番茄 Tomato ABC-C6, ABC-G33 TRV 根系分泌 The root secretion 根结线虫减少 Reduced nematodes [51] 茄子 Eggplant NBS-LRR TRV 根结线虫 Nematodes 抗病性减弱 Attenuates disease resistance [52] 辣椒 Pepper CaWRKY27 TRV 盐胁迫 Salt stress 抗性减弱 Attenuates resistance [53] 番茄 Tomato GSTU43 TRV 抗低温性 Chilling resistance 抗性减弱 Attenuates resistance [54] 辣椒 Pepper CaDIF1, CaDIS1 TRV 脱落酸和干旱胁迫 ABA and drought stress 气孔增大,蒸腾增加 Stomata and transpiration increases [55] 番茄 Tomato MYB80 TRV 抗低温 Chilling stress 抗低温减弱 Attenuates chilling resistance [56]