绿盲蝽雷帕霉素靶蛋白的克隆、抗体制备及在蜕皮激素诱导下的应答

谭永安,赵旭东,姜义平,赵静,肖留斌,郝德君

Cloning, Preparation of Antibody and Response Induced by 20-Hydroxyecdysone of Target of Rapamycin in Apolygus lucorum

TAN YongAn,ZHAO XuDong,JIANG YiPing,ZHAO Jing,XIAO LiuBin,HAO DeJun

图9 原核表达质粒pCzn1-AlTOR诱导表达蛋白的SDS-PAGE分析（A）和pCzn1-AlTOR表达蛋白纯化的SDS-PAGE分析（B） M:蛋白分子量标准物Protein molecular weight marker。A:1:未经IPTG诱导的pCzn1-AlTOR表达蛋白pCzn1-AlTOR expressed protein without induced by IPTG;2:IPTG诱导的pCzn1-AlTOR表达蛋白pCzn1-AlTOR expressed protein induced by IPTG;3:重组质粒pCzn1-AlTOR表达蛋白上清Supernatant of pCzn1-AlTOR expressed protein induced by IPTG;4:重组质粒pCzn1-AlTOR表达蛋白沉淀Precipitate of pCzn1-AlTOR expressed protein induced by IPTG。B:1:未纯化的包涵体Unpurified inclusion body;2:未与Ni柱结合的杂蛋白Uncombined heterozygous protein with Ni NTA column;3:与Ni柱结合的目的蛋白Combined target protein with Ni NTA column

Fig. 9 Expression SDS-PAGE analysis induced by IPTG (A) of pCzn1-AlTOR and SDS-PAGE of purified pCzn1-AlTOR fusion protein (B)