绿盲蝽雷帕霉素靶蛋白的克隆、抗体制备及在蜕皮激素诱导下的应答

谭永安,赵旭东,姜义平,赵静,肖留斌,郝德君

Cloning, Preparation of Antibody and Response Induced by 20-Hydroxyecdysone of Target of Rapamycin in Apolygus lucorum

TAN YongAn,ZHAO XuDong,JIANG YiPing,ZHAO Jing,XIAO LiuBin,HAO DeJun

表1 本研究所用的引物

Table 1 Primer sequences used in this study

引物名称/目的 Primer name/Purpose 序列 Primer sequence (5′ to 3′) 克隆Cloning 5′-AlTOR-1 AACGACTGGGCAAGAA 5′-AlTOR-2 CAAGACGTTTGGGTAGCA 3′-AlTOR-1 AGAGACTCAGTCGCTCACTATCAAGA 3′-AlTOR-2 CATTGAGACCACACCCCAGACACA AlTOR-F ATGGTGGAAAAATATGCTCG AlTOR-R GCTAGCCAGTATACAACG 表达谱分析qRT-PCR RT-AlTOR-F GGGGTCGTCAACTGGCAGAA RT-AlTOR-R CCTCCTTCTTCTTGGGTGCGA Al-β-Actin-F ACCTGTACGCCAACACCGT Al-β-Actin-R TGGAGAGAGAGGCGAGGAT