苹果蠹蛾几丁质脱乙酰基酶2的基因克隆、表达模式和分子特性

石国良,武强,杨念婉,黄聪,刘万学,钱万强,万方浩

Gene Cloning, Expression Pattern and Molecular Characterization of Chitin Deacetylase 2 in Cydia pomonella

SHI GuoLiang,WU Qiang,YANG NianWan,HUANG Cong,LIU WanXue,QIAN WanQiang,WAN FangHao

图1 CpCDA2a和CpCDA2b基因结构和蛋白结构域分析 A:CpCDA2基因结构和CpCDA2a、CpCDA2b的选择性剪切,白色框和黑色线段分别表示外显子和内含子,黑色实心方框表示编码序列,ATG和TGA分别表示起始和终止密码子Gene structure of CpCDA2 and alternatively spliced CpCDA2a, CpCDA2b, white boxes and black lines indicate exons and introns, black solid boxes indicate coding sequence, ATG and TGA indicate start and end codons;B:红色箭头为信号肽,绿色菱形为几丁质结合域,黄色六边形为低密度脂蛋白受体域,紫色圆柱为催化功能域The red arrow indicates signal peptide (SP), green diamond indicates chitin binding domain (ChBD), yellow hexagon indicates low density lipoprotein receptor class A domain (LDLa), purple cylinder indicates chitin deacetylase catalytic domains (CDA)

Fig. 1 Genome structure and protein domain analysis of CpCDA2a and CpCDA2b