苹果蠹蛾几丁质脱乙酰基酶2的基因克隆、表达模式和分子特性
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石国良,武强,杨念婉,黄聪,刘万学,钱万强,万方浩
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Gene Cloning, Expression Pattern and Molecular Characterization of Chitin Deacetylase 2 in Cydia pomonella
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SHI GuoLiang,WU Qiang,YANG NianWan,HUANG Cong,LIU WanXue,QIAN WanQiang,WAN FangHao
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表1 本研究中所用引物信息
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Table 1 Information of primers used in this study
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| 基因名称 Gene name | 引物序列 Primer sequence (5′-3′) | 产物长度 Product length (bp) | 用途 Application | | CpCDA2a/CpCDA2b | F: CAGTCCTCGGCGAGCGTTAT | 1858/1841 | cDNA克隆
cDNA cloning | | R: CCACGCAAAAGGCATTCCAA | | CpCDA2a | qFc: TGTTGCTTGCTTGCTTCGTTAG | 176 | 荧光定量PCR
RT-qPCR | | qRa: GTTCTCGCCGCCCTTGTC | | CpCDA2b | qFc: TGTTGCTTGCTTGCTTCGTTAG | 233 | | qRb: CCAGTCGCAGGTTTGTTTATCG | | Cpβ-tubulin | qF: GCGGGAACCAGATTGGAGCTAA | 267 | | qR: ACTGGCCGAACACGAAGTTGTC |
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