木尔坦棉花曲叶病毒“C4 ORF”编码蛋白对病毒致病性的影响

郑信诗,尚鹏祥,李景远,丁新伦,吴祖建,张洁

Effects of Proteins Encoded by “C4 ORFs” of Cotton Leaf Curl Multan Virus on Viral Pathogenicity

ZHENG XinShi,SHANG PengXiang,LI JingYuan,DING XinLun,WU ZuJian,ZHANG Jie

图4 CLCuMuV及其突变体侵染性克隆接种本氏烟植株 A:本氏烟症状Symptoms of N. benthamiana plants。B:接种21 d后本氏烟中DNA-A的Southern blot检测Southern blot analysis of the DNA-A accumulation in N. benthamiana plants at 21 dpi。C:接种21 d后本氏烟中DNA-β的Southern blot检测Southern blot analysis of the DNA-β accumulation in N. benthamiana plants at 21 dpi;Total DNA:总DNA。D:接种14 d后本氏烟中C4蛋白的Western blot检测Western blot analysis of the C4 protein accumulation in N. benthamiana plants at 14 dpi;Rubisco:核酮糖-1,5-二磷酸羧化酶 Ribulose-1,5-bisphosphate carboxylase

Fig. 4 N. benthamiana plants inoculated by infectious clones of CLCuMuV and the mutants