木尔坦棉花曲叶病毒“C4 ORF”编码蛋白对病毒致病性的影响

郑信诗,尚鹏祥,李景远,丁新伦,吴祖建,张洁

Effects of Proteins Encoded by “C4 ORFs” of Cotton Leaf Curl Multan Virus on Viral Pathogenicity

ZHENG XinShi,SHANG PengXiang,LI JingYuan,DING XinLun,WU ZuJian,ZHANG Jie

图2 PVX载体介导的CLCuMuV 3个“C4 ORF”接种本氏烟植株 A:本氏烟症状Symptoms of N. benthamiana plants。B:接种25 d后本氏烟叶柄长度Petiole length of N. benthamiana plants 25 days post inoculation (dpi);数据为平均值±标准误,柱上**表示在P<0.01水平差异显著All values are shown as the mean±SE, ** on the column indicates significant difference at P<0.01 level。C:Western blot检测接种25 d后本氏烟中的C4蛋白Western blot analysis of the C4 protein accumulation in N. benthamiana plants at 25 dpi;Rubisco:核酮糖-1,5-二磷酸羧化酶 Ribulose-1,5-bisphosphate carboxylase

Fig. 2 N. benthamiana plants inoculated by three “C4 ORFs” of CLCuMuV using the PVX vector