木尔坦棉花曲叶病毒“C4 ORF”编码蛋白对病毒致病性的影响

郑信诗,尚鹏祥,李景远,丁新伦,吴祖建,张洁

Effects of Proteins Encoded by “C4 ORFs” of Cotton Leaf Curl Multan Virus on Viral Pathogenicity

ZHENG XinShi,SHANG PengXiang,LI JingYuan,DING XinLun,WU ZuJian,ZHANG Jie

表1 本研究中所用引物序列

Table 1 Primers used in this study

引物用途及名称 Application and name of primer 引物序列a Primer sequence (5′- 3′) 引物在病毒基因组中的位置b Position of primers in viral genome PVX异源表达For PVX heterologous expression C4-L-F cagctagcatcgattcccgggATGATTTCTCAAATTACGATAATGC 2694-2670 C4-M-F cagctagcatcgattcccgggATGCCATTTGGAGACACTAT 2673-2654 C4-S-F cagctagcatcgattcccgggATGGGAGCCCTCATCTCCAT 2430-2411 C4-R cttatcggcggtcgacccgggCTAGTTCCTTAATGACTCTAAGAGC 2128-2152 PVX-F CCCTATCAAGCTTATCGGCGGTC -- PVX-R CATTGCCGATCTCAAGCCACTCTC -- 亚细胞定位For subcellular localization C4-L-221-F ggggacaagtttgtacaaaaaagcaggcttcATGATTTCTCAAATTACGATAATG 2694-2671 C4-221-R ggggaccactttgtacaagaaagctgggtcGTTCCTTAATGACTCTAAGAGCC 2131-2153 M13-F TGTAAAACGACGGCCAGT -- M13-R CAGGAAACAGCTATGACC -- Gateway-F GGGCTGGCAAGCCACGTTTGGTG -- Gateway-R CCGGGAGCTGCATGTGTCAGAGG -- 侵染性克隆For infectious clones CLCuMuV-L-F acgccaagctggcgcgccaagcttGATCCATTGTTAAACGAATTTCCTGATACG 123-152 CLCuMuV-L-R TTTAACAATGGATCCCACATgtttgaatttgaaacttagtgcgca 136-117 CLCuMuV-R-F ATGTGGGATCCATTGTTAAACGAATTTCC 117-145 CLCuMuV-R-R agaattcgagctcggtacccgggcacatGTTTGAATTTGAAACTTAGTGCGCA 116-92 pBIN-F GTATGTTGTGTGGAATTGTGAGCGGATAAC -- pBIN-R CACTGGCCGTCGTTTTACAACGTCGTGAC -- C4L-i2o-R-pBIN-F TCACATTTCAAAATCCTCACGCTCCAAAAAG 2692-2719 C4L-i2o-L-pBIN-R GGAGCGTGAGGATTTTGAAATGTGATCTCAAATTACGATAATGCCATTTG 2713-2692, 2688-2664 C4S-G2O-R-pBIN-F ACATCTTCGTGTAACTCTCTGCAGAC 2430-2452 C4S-G2O-L-pBIN-R TCTGCAGAGAGTTACACGAAGATGTGAGCCCTCATCTCCATGTGCTCATC 2451-2428, 2424-2402 CLCuMuV-RBamH1-pBIN-F TTCAAATTCAAACATGTGGGATCCATTGTTAAACGAATTTCCTG 104-147 CLCuMuV-CPprobe-F GCAGCCCATACACCAGCCGTGTTGC 347-371 CLCuMuV-CPprobe-R GTTCCTTACTCGCATATTGACCACC 862-838 CLCuMuV-BETAprobe-F GTCCCACTGCTGGTATTGACTTGATTTG 37-64 CLCuMuV-BETAprobe-R CCATGAAATAAGCAGATATGACGAGGAGC 567-539