利用CRISPR/Cas9技术编辑BADH2-1/BADH2-2创制香米味道玉米新种质

张翔,史亚兴,卢柏山,武莹,刘亚,王元东,杨进孝,赵久然

Creation of New Maize Variety with Fragrant Rice Like Flavor by Editing BADH2-1 and BADH2-2 Using CRISPR/Cas9

ZHANG Xiang,SHI YaXing,LU BaiShan,WU Ying,LIU Ya,WANG YuanDong,YANG JinXiao,ZHAO JiuRan

附图4 T2代Zmbadh2-2纯合突变株系突变形式及2-AP含量分析 A:ZmBADH2-2靶点设计示意图。突变检测引物见电子附表1。B:BH-2-3和BH-2-4 2个ZmBADH2-2编辑纯合突变株系靶点突变形式。C:水稻品种日本晴、稻花香,玉米自交系B104,Zmbadh2-1/Zmnadh2-2双突变株系BH02及2个ZmBADH2-2编辑株系的2-AP含量。小写字母代表各株系间2-AP含量在0.01水平差异极显著

Supplementary Fig.4 Mutations and 2-AP content of Zmbadh2-2 mutants A: Diagram of the sgRNA design of ZmBADH2-2. Primers used to detect mutation were shown in supplementary table 1. B: Mutations of two gene editing line on ZmBADH2-2. C: 2-AP content of Nipponbare, Daohuaxiang, B104 maize inbred line, Zmbadh2-1/Zmnadh2-2 double mutant line BH02 and two gene editing line of Zmbadh2-2 mutant in B104 background. Lower case letters represented significant difference (P<0.01) in 2-AP contents of different gene editing lines