利用CRISPR/Cas9技术编辑BADH2-1/BADH2-2创制香米味道玉米新种质

张翔,史亚兴,卢柏山,武莹,刘亚,王元东,杨进孝,赵久然

Creation of New Maize Variety with Fragrant Rice Like Flavor by Editing BADH2-1 and BADH2-2 Using CRISPR/Cas9

ZHANG Xiang,SHI YaXing,LU BaiShan,WU Ying,LIU Ya,WANG YuanDong,YANG JinXiao,ZHAO JiuRan

图2 ZmBADH2-1和ZmBADH2-2靶点设计（A）及突变类型（B）分析 下划线表示靶点序列,红色字体表示PAM序列,绿色字体代表碱基插入,-:碱基缺失,蓝色字体表示碱基替换

Fig. 2 Diagram of the sgRNA design (A) and mutation analysis (B) on ZmBADH2-1 and ZmBADH2-2 The nucleotides with underline represented target sequence, the nucleotides with red font represented PAM sequence, the nucleotides with green font indicated bases of insertion, -: Base deletions, blue words indicated base substitutions