水稻CSC11介导干热风/干旱诱导的钙信号调控雄蕊发育

任志杰,李倩,孙钰佳,孔冬冬,刘良玉,侯聪聪,李乐攻

OsCSC11 Mediates Dry-Hot Wind/Drought-Induced Ca2+ Signal to Regulate Stamen Development in Rice

REN ZhiJie,LI Qian,SUN YuJia,KONG DongDong,LIU LiangYu,HOU CongCong,LI LeGong

图6 基因编辑靶点设计和oscsc11突变体鉴定 A:OsCSC11的结构和靶点位置,蓝色字体为靶序列,红色字体为PAM序列,水平的箭头表示突变体鉴定引物所在的位置;B:CRISPR/Cas9基因编辑阳性植株的突变类型,红色小写字体表示插入碱基,绿色虚线表示缺失碱基,+1表示插入一个碱基,-2表示缺失2个碱基;C:纯合突变体测序峰图,红色箭头表示碱基插入的位置

Fig. 6 Diagram of the sgRNA design and identification of oscsc11 mutants A: The diagram of OsCSC11 and the sgRNA. The target and PAM sequences are in blue and red respectively. B: Identification of mutation types in gene-edited plants. The inserted bases are shown in red and the deleted bases are indicated by green dotted lines. +1 means 1-bp-insertion and -2 means 2-bp-deletion. C: The sequencingmap of homozyg mutants. The red arrows indicate insertion or deletion positions