水稻CSC11介导干热风/干旱诱导的钙信号调控雄蕊发育

任志杰,李倩,孙钰佳,孔冬冬,刘良玉,侯聪聪,李乐攻

OsCSC11 Mediates Dry-Hot Wind/Drought-Induced Ca2+ Signal to Regulate Stamen Development in Rice

REN ZhiJie,LI Qian,SUN YuJia,KONG DongDong,LIU LiangYu,HOU CongCong,LI LeGong

图5 OsCSC11的电生理学分析 A:全细胞记录方式检测表达OsCSC11的蛙卵和对照组注射等体积水的蛙卵在高渗胁迫时产生的电流,电压钳制在-100 mV,横坐标表示记录时间,纵坐标表示电流大小,实线表示电流示踪,虚线表示0 μA电流;B:统计分析A图中记录到的被高渗溶液激活的电流最大值,n>3;C:OsCSC11和OsCSC11ΔTM1-3蛋白的跨膜结构预测;D:OsCSC11和OsCSC11ΔTM1-3蛙卵细胞中的定位以及电生理活性检测,注射水的蛙卵作为对照;E:统计分析图B中钳制电压为-140 mV时的电流最大值,n>5;F:OsCSC11和OsCSC11ΔTM1-3的离子选择性分析,n>5;B、E和F图中的数值由平均值±标准差表示

Fig. 5 Electrophysiological analysis of OsCSC11 A: Whole-cell recording of OsCSC11-expressing Xenopus laevis oocytes and the water-injected control oocytes perfused with hyperosmotic solution containing 500 mM D-mannitol. The holding potential was at -100 mV. The X-axis represents the recording time and the Y-axis represents the current. The solid and dotted lines indicate traces and 0 μA current, respectively. B: Current amplitudes evoked by the application of hyperosmotic stress (n> 3) C: Prediction of transmembrane helices in OsCSC11 and OsCSC11ΔTM1-3. D: Electrophysiological activity of OsCSC11and OsCSC11ΔTM1-3 in oocytes, and the oocytes injected with water as a control. E: Statistic analysis of the maximum currents at holding potential of -140 mV as in B (n > 5). F: Selectivity analysis of OsCSC11 and OsCSC11ΔTM1-3 (n >5). The data are shown as mean ± SD in B, E and F