水稻CSC11介导干热风/干旱诱导的钙信号调控雄蕊发育

任志杰,李倩,孙钰佳,孔冬冬,刘良玉,侯聪聪,李乐攻

OsCSC11 Mediates Dry-Hot Wind/Drought-Induced Ca2+ Signal to Regulate Stamen Development in Rice

REN ZhiJie,LI Qian,SUN YuJia,KONG DongDong,LIU LiangYu,HOU CongCong,LI LeGong

图3 OsCSC11的表达模式 A:qRT-PCR检测水稻不同组织OsCSC11的表达;根、茎、叶、叶鞘来自抽穗前的野生型水稻,数字:小穗抽出的长度（cm）;B—E:稳定表达ProCSC11-GUS转基因水稻的GUS染色,B:萌发1、2、3、4和7 d的小苗,标尺为0.5 cm;C:授粉时期的颖花,标尺为0.1 cm;D:成熟的雄蕊,标尺为0.05 cm;E:成熟花粉粒,标尺为100 μm

Fig. 3 The expression pattern of OsCSC11 A: Expression of OsCSC11 in various organs analyzed by qRT-PCR; Roots, culms, leaves, and sheathes were harvested from WT plants before heading. Anthers and pistils from spikelet hulls were collected two days before fertilization. Numbers of 1, 5, 10, 15 and 20 stand for different lengths (cm) of the heading panicles. B-E: GUS staining of various tissues from ProOsCSC11-GUS transgenic plants. B: Seedlings grow for 1 day, 2 day, 3 day, 4 day and 7 day were represented (scale bar=0.5 cm); C: Rice floret during pollination (scale bar=0.1 cm); D: Anthers (scale bar=0.05 cm); E: Pollens (scale bar=100 μm)