水稻CSC11介导干热风/干旱诱导的钙信号调控雄蕊发育

任志杰,李倩,孙钰佳,孔冬冬,刘良玉,侯聪聪,李乐攻

OsCSC11 Mediates Dry-Hot Wind/Drought-Induced Ca2+ Signal to Regulate Stamen Development in Rice

REN ZhiJie,LI Qian,SUN YuJia,KONG DongDong,LIU LiangYu,HOU CongCong,LI LeGong

表1 本研究所用的引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途 Purpose CSC11-BamHⅠ-F CGGGATCCATGGGGCCGACCGCGCCGCCGCCGGACGCCG 蛙卵表达载体构建 Vector construction for oocyte expression CSC11-EcoRⅠ-R GGAATTCTCAGGATTGATACAGGCTCCAATCC CSC11ΔTM1-3-BamHⅠ-F CGGGATCCATGGAGGACGCCCTTCGCA ProCSC11- Hind Ⅲ-F CCCAAGCTTTATAGAATGGGTCATCATAGCA p1300-proCSC11-GUS表达载体构建 Construction of of p1300-proCSC11-GUS vector ProCSC11- BamHⅠ-R CGGGATCCCGCCGGGGGACGGGGACGTGAC CSC11- EcoRⅠ-F GGAATTCATGGGGCCGACCGCGCCGCCGCCGGACGCCG GFP融合表达载体 GFP-CSC11 expression vector CSC11-BamHⅠ-R CGGGATCCGGATTGATACAGGCTCCAATCC CSC11ΔTM1-3-EcoRⅠ-F GGAATTCATGGAGGACGCCCTTCGCA Target-F GCGGCGGGGAGCCGGAGGCG 基因敲除载体 Gene editing Target-R CGCCTCCGGCTCCCCGCCGC CSC11-CRI-F ACCTCGCGTGATCTAGCCCCACC 靶点检测及测序引物 Target detection and sequencing primer CSC11-CRI-R GCTTCTCTCAAGCTGGAGCTCC 11-qRT-F GGGCATTCCCAAGACGCT qRT-PCR检测引物 Primers used for qRT-PCR 11-qRT-R CCAAGAAATCCTGTTCCGCA OsACTIN1-F TCCATCTTGGCATCTCTCAG OsACTIN1-R GTACCCGCATCAGGCATCTG