小飞蓬捕光叶绿素结合蛋白基因CcLhca-J9克隆及表达分析

李祖任,罗丁峰,柏浩东,徐晶晶,韩进财,徐强,王若仲,柏连阳

Cloning and Expression Analysis of Light Harvesting Chlorophyll a/b Protein Gene CcLhca-J9 in Conyza canadensis

LI ZuRen,LUO DingFeng,BAI HaoDong,XU JingJing,HAN JinCai,XU Qiang,WANG RuoZhong,BAI LianYang

表1 CcLhca-J9克隆及实时荧光定量PCR表达引物

Table 1 The primers used for CcLhca-J9 cloning and RT-qPCR

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 引物用途 Use of primers CcJ9-1 Forward: GAGYTGCGGYATCBCCGCCGT Reverse: GTTGTGCCATGGRTCAGCCAA 核心片段扩增 Fragment cloning CcJ9-F2 Forward: CGGATTTGACCCACTTCGTCT 5′ RACE扩增 5′ cDNA end amplification CcJ9-R2 Reverse: TATTGCCAGTATGGTGGGTAGGGT 5′ RACE扩增 5′ cDNA end amplification CcJ9-R3 Reverse: GGCAGCCCATTCTTGTGCTTTTAC 5′ RACE扩增 5′ cDNA end amplification CcJ9-F3 Forward: GGCAACTGGGTAAAAGCACAAGAAT 3′ RACE扩增 3′ cDNA end amplification CcJ9-F4 Forward: AAGACCCAAAAACATTCGCAGAGTA 3′ RACE扩增 3′ cDNA end amplification qR-J9 Forward: CATTCTTGTGCTTTTACCCAG Reverse: GAGGTTTAAAGAGTCCGAGCT 实时荧光定量PCR RT-qPCR ACTIN Forward: CCGATCCAGACGCTGTATTT Reverse: TGCTGATCGTATGAGCAAGG 实时荧光定量PCR RT-qPCR