蜜蜂球囊菌中长链非编码RNA的调控作用
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周丁丁,范元婵,王杰,蒋海宾,祝智威,范小雪,陈华枝,杜宇,周紫彧,熊翠玲,郑燕珍,付中民,陈大福,郭睿
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Regulatory Function of Long Non-Coding RNAs in Ascosphaera apis
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ZHOU DingDing,FAN YuanChan,WANG Jie,JIANG HaiBin,ZHU ZhiWei,FAN XiaoXue,CHEN HuaZhi,DU Yu,ZHOU ZiYu,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui
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表1 本研究使用的引物
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Table 1 Primers used in this study
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核酸ID
Nucleic acid ID | 引物名称
Primer name | 引物序列
Primer sequence (5′-3′) | 产物大小
Product size (bp) | | MSTRG.6443.1 | F | AAAATGAAAAGGCAAATC | 210 | | R | AAGGTCAAGAAGCACAAG | | MSTRG.2135.1 | F | AGAAGCAGCAAGGAAGTCG | 128 | | R | GGCAGGGCAATAACAAAAC | | MSTRG.2134.1 | F | ACTCACTCTCTGCCCCTC | 238 | | R | CCCATTTATTTGCTACCG | | MSTRG.3422.1 | F | AACCGAAAAACTCAAGGA | 160 | | R | TCGCAATCAGACATCAAA | | MSTRG.2302.1 | F | TGTCTGTCCGTTCGTCCTT | 139 | | R | CAGCGTAGCGTTGTGTAGT | | MSTRG.5023.1 | F | CAAACCCAGATTTATTCC | 110 | | R | TACCTTTCCTCCTTACGA | | MSTRG.5584.1 | F | GAAGACATTCAATCCAACAC | 184 | | R | AGCCACACACTTATCCACTA | | MSTRG.1870.1 | F | GCCTCTTTTCGGTTTGCT | 158 | | R | CGTTCTTGCTCGTGTCGT | | MSTRG.1614.1 | F | AAATCGGAACGGTGAGGGA | 115 | | R | GGCAGTGACCAAAGGCAAA | | gene1602 | F | AGCCCCGCTACCAAACTC | 110 | | R | CTGTCCCTCATCGCCATA | | gene1970 | F | AGTTCTCTTACTGGCGTCTTTG | 100 | | R | CTTTCACTTGCTGGGCTTTCTC | | gene2674 | F | TGGCTTCCTACACAAACCT | 233 | | R | TTCCTTATTCACCCGCTCC | | gene4126 | F | ATGAACAATGTCAAGGAAGC | 320 | | R | TCTGGAAAGAGTGTGGGGAG | | gene4125 | F | CCAAACAGACAAATGGCTAA | 120 | | R | TCGGGAAAGATGATTGAGAA | | gene5384 | F | TATGGTTATGCCTATGGTTT | 122 | | R | GATGTGGGGTATCTCGTGTT | | gene1986 | F | CATTGATACCGATACAGAGAC | 237 | | R | GAGATAACATTGACAACGCCT | | gene989 | F | CCTCTTCCCACACTCTCACT | 236 | | R | AGACTCCAAAACCTGCTCCT | | miR-281-y | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCAAAGAGA | 72 | | F | ACACTCCAGCTGGGTGTCATGGAGTTGC | | miR-13-y | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGCTCATC | 73 | | F | ACACTCCAGCTGGGTATCACAGCCATTTT | | miR-11980-y | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCTGCCAA | 68 | | F | ACACTCCAGCTGGGGGGAACGGGC | | miR-6057-y | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCATTTGTT | 72 | | F | ACACTCCAGCTGGGTTTGTGACTGTAAC | | miR-1-z | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGATACATAC | 72 | | F | ACACTCCAGCTGGGTGGAATGTAAAGAA | | miR-9-z | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCATACAG | 73 | | F | ACACTCCAGCTGGGTCTTTGGTTATCTAG | | miR-13-x | L | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCATTCCAC | 72 | | F | ACACTCCAGCTGGGACATCAAATTGGTT | | actin (gene6001) | F | GCTACTTCCCATCATTCGTC | 92 | | R | CCCAATCTGTGACAATCCC | | - | Universal R | CTCAACTGGTGTCGTGGA | |
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