图3. 农杆菌及拟南芥转化株中BraERF023a的分子鉴定
a：分别采用BraERF023a特异扩增引物及BraERF023a与载体序列组合引物对农杆菌阳性克隆进行菌液PCR的检测结果,M：DNA marker,1—6：6个阳性克隆特异引物PCR检测结果,1′—6′：6个阳性克隆组合引物PCR检测结果。b：分别采用BraERF023a特异扩增引物及BraERF023a与载体序列组合引物对拟南芥转化株的单株PCR检测结果,M：DNA marker,1—10：10个单株特异引物PCR检测结果,1′—10′：10个单株组合引物PCR检测结果。c：不同BraERF023a过表达拟南芥转化株系中BraERF023a表达水平的qRT-PCR检测结果,CK：对照组,L3/L4/L6/L8/L9：BraERF023a过表达拟南芥株系

Fig. 3. Identification of BraERF023a in Agrobacterium tumefaciens and transgenic lines in Arabidopsis
a: Identification of Agrobacterium tumefaciens with BraERF023a by PCR used the specific primers and combined primers, respectively. M: DNA marker, 1-6 indicated PCR identification used the specific primers, 1′-6′ indicated PCR identification used the combined primers. b: Identification of individual Arabidopsis plant with BraERF023a by PCR used the specific primers and combined primers, respectively. M: DNA marker, 1-10 indicated PCR identification of individual plants used the specific primers, 1′-10′ indicated PCR identification of individual plant used the combined primers. c: Expression levels of BraERF023a in differential overexpression BraERF023a transgenic Arabidopsis lines detected by qRT-PCR, CK: Wild-type control, L3/L4/L6/L8/L9 indicated the five independent transgenic lines