花椰菜BraERF023a的克隆及在响应盐和干旱胁迫中的功能

李慧,韩占品,贺丽霞,杨亚苓,尤书燕,邓琳,王春国

Cloning and Functional Analysis of BraERF023a Under Salt and Drought Stresses in Cauliflower (Brassica oleracea L. var. botrytis)

LI Hui,HAN ZhanPin,HE LiXia,YANG YaLing,YOU ShuYan,DENG Lin,WANG ChunGuo

表1 BraERF023a克隆、载体构建和定量RT-PCR分析引物序列

Table 1 Primers used for BraERF023a cloning, vector construction and qRT-PCR assay

引物名称 Primer name 引物序列 Primer sequence (5′-3′) BraERF023a-F ATGCTGAGTACAGTGAGTGAAACC BraERF023a-R TCACAGACACGCCATGAACTG Nco I-BraERF023a-F CCATGGATGCTGAGTACAGTGAGTG BstE II-BraERF023a-R GGTCACCTCACAGACACGCCAT qBraERF023a-F CCACCACTTACCAACGAAC qBraERF023a-R AAGATCCGAGCCAAATCCG 35S-F AACAGAACTCGCCGTAAAG BraActin-F GCTCCTCTTAACCCAAAGGC BraActin-R CACACCATCACCAGAATCCAGC AtActin-F GGTAACATTGTGCTCAGTGGTGG AtActin-R AACGACCTTAATCTTCATGCTGC