重组GSTA3蛋白对福美双诱导的肉鸡TD中抗凋亡基因BAG-3表达的影响

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Effect of Recombinant GSTA3 Protein on Expression of the Anti-Apoptotic Gene BAG-3 in Thiram-Induced Tibial Chondrodysplasia

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表2 Real-time PCR所用引物

Table 2 Primer design for Real-time PCR

基因Gene 引物序列（5′→3′）Primer Sequence（5′→3′） 片段Size (bp) 退火温度Annealing temperature (℃) BAG-3 F: TTCTGATTCTCCAACGGTTT R: TTCATGGATCACAGGAATTG 100 55 18S rRNA F：TTCCGATAACGAACGACAC R：GACATCTAAGGGCATCACAG 139 55