GnIH通过p38MAPK信号通路对猪卵巢颗粒细胞自噬与凋亡的影响
张鑫,霍孔林,宋星星,张多妮,胡文,胡传活(
),李珣()
),李珣()
Effects of GnIH on Autophagy and Apoptosis of Porcine Ovarian Granulosa Cells via p38MAPK Signaling Pathway
Xin ZHANG,KongLin HUO,XingXing SONG,DuoNi ZHANG,Wen HU,ChuanHuo HU(),Xun LI()
),Xun LI()
图5. 用p38激活剂孵育,用不同剂量GnIH（0、10-6、10-8、10-10、10-12）处理pGCs,Western blot检测caspase-3、Bax和Bcl-2的免疫印迹及统计学分析
用ImageJ进行灰度值分析。数值为平均值±S.E,与对照组比较,星号表示显著差异。*P<0.05;**P<0.01
Fig. 5. After incubated with p38 activator, RFRP-3 concentration gradient (0, 10-6, 10-8, 10-10, 10-12) was treated with pGCs.Western blot caspase-3,Bax and Bcl-2 and statistical analysis (n=3)
Densitometric quantification was performed using ImageJ with GAPDH as the internal control for normalization. Values are mean±S.E. Compared with control group, asterisk indicates significant difference. *P<0.05; **P<0.01
