柑橘溃疡病抗性SNP验证及其相关钙依赖性蛋白激酶基因诱导表达

彭蕴,雷天刚,邹修平,张靖芸,张庆雯,姚家欢,何永睿,李强,陈善春

Verification of SNPs Associated with Citrus Bacterial Canker Resistance and Induced Expression of SNP-Related Calcium-Dependent Protein Kinase Gene

Yun PENG,TianGang LEI,XiuPing ZOU,JingYun ZHANG,QingWen ZHANG,JiaHuan YAO,YongRui HE,Qiang LI,ShanChun CHEN

表1 SNP位点及高分辨率熔解曲线分型PCR引物

Table 1 SNP loci and the PCR primers used for SNP genotyping by HRM

SNP编号 SNP number SNP位点 SNP loci 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) HP02 GGTTTTT GGTGTTT AGAGCTGCCTTAGTGGTGTC CAGTCTCTACTGCCCCGTTC HP17 ATATCCC ATAGCCC ACCAGTTACTCTCCGTCCGA TTAGGGTTTGGCGAGGACG HP18 GATCGAA GATTGAA CAGAACATTATCCGGCATGGG CCTGAATGAGAGGTGCTGATTT HP31 GCCATAT GCCGTAT TGGCAGAATGTTGGGTAGGG CCACTCAAGAGCCGCCTTAT HP40 ACGCTCA ACGTTCA ATCCTCCTTCTCCCGTCACC TGGGTTTGGTCATGTCCTTCC HP42 ATTCTCC ATTTTCC ATCAAGTAACACACTCCCTTTACG TGAGACTTTATATTTGTGATTGCGA HP54 TGCCCTT TGCTCTT GGGTTCCCCGTTTCAGATGT CCACCTCCGATGGAAAGAAGT HP75 TTACTCC TTATTCC TCCTTCTGCCCGGATGATGA TCCTTCTGCCCGGATGATGA HP85 TTGCCC TTGTCC CACAAGTCACAAGCCGCAAT AAGTACGATTGCCCGAGGTC HP87 ACAGTAG ACATTAG ATCAATGGGGTCACCAACCC ATTGGTGGTGGCGTTAGTCC HP108 CATACCAA CATATCAA TGAAAGTGAACTTCAACGATGC TCGTTGAGGAGAGGTCCAAGA HP127 CTCTTTCC CTCTCTCC TCGGCTTTTGTCGCGTTTAC TAGCTGACACGACACCGTTT HP128 ATCCCTTT ATCACTT TGGATCCCACCATGGAATTGT AGCGATGAGTTTAAGGAGAAGGG HP170 AACTCGA AACCCGA ATGGATCCTTTTGAGAGAAGAAAACTGT TGTCATTCTTGCCTTTTCCTTTCT