基于亲本对条锈病敏感性预测小麦杂交种的抗性

周天宇,李姜玲,杨澜,阮仁武,杨宇衡,李中安

The Resistance Prediction of Wheat Hybrids Based on the Sensibility of Their Parents to Stripe Rust

TianYu ZHOU,JiangLing LI,Lan YANG,RenWu RUAN,YuHeng YANG,ZhongAn LI

表2 小麦条锈菌生理小种特异性引物序列

Table 2 The specific primer sequences for the physiological races of Pst

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 退火温度 Anneal temperature (℃) CYR23 S360[33] S413 F: AAGCGGCCTC R: GGTGGTCCAAG 50 CYR31 CY31SP-1[34] CY31SP-2 F: GCTACGTCAAGATGCGATACACC R: TGTCAGAAGCAAGTGGTAAACTAGG 50 CYR33 CYR33SP-1[35] CYR33SP-2 F: TGTCGTCTCGCCAATCTTT R: GCGGGTGTCAGTTTCTCC 50 CYR34 V26SP-1[36] V26SP-2 F: CTGTAAAGCGGATAAAGGAA R: CATAAGAGCCACACTTGACC 57 Pst elongation factor Pst_EF F: TTCGCCGTCCGTGATATGAGACAA R: ATGCGTATCATGGTGGTGGAGTGA 55