基于亲本对条锈病敏感性预测小麦杂交种的抗性

周天宇,李姜玲,杨澜,阮仁武,杨宇衡,李中安

The Resistance Prediction of Wheat Hybrids Based on the Sensibility of Their Parents to Stripe Rust

TianYu ZHOU,JiangLing LI,Lan YANG,RenWu RUAN,YuHeng YANG,ZhongAn LI

表1 小麦抗条锈病基因的引物序列

Table 1 The primer sequences for stripe rust resistance genes

Yr 基因 Yr genes 引物名称 Primer name 引物序列 Primer sequence (5′-3′) 退火温度 Anneal temperature (℃) 片段大小 Fragment size (bp) Yr5 Yr5-InsertionF[26] Yr5-InsertionR CTCACGCATTTGACCATATACAACT TATTGCATAACATGGCCTCCAGT 52 +1281 Yr9 AF1[27] AF4 F: GGAGACATCATGAAACATTTG R: CTGTTGTTGGGCAGAAAG 46 +1500 Yr10 Yr10F1[27] Yr10R1 TTGGAATTGGCGACAAGCGT GTGATGATTACCCACTTCCTC 50 +755 Yr15 Y15K1_F2[28] uhw301R GGAGATAGAGCACATTACAGAC TTTCGCATCCCACCCTACTG 55 +992 W_2F TGCACGCGGATATTAGGTAGG 55 +2014 W_2R TGATGAAGAGGACCAACGCA Yr17 VENTRIUP[27] LN2 AGGGGCTACTGACCAAGGCT TGCAGCTACAGCAGTATGTACACAAAA 55 +262 Yr18 L34DINT9F[27] L34PLUSR TTGATGAAACCAGTTTTTTTTCTA GCCATTTAACATAATCATGATGGA 45 +517 Yr26 WE173F[27] WE173R GGGACAAGGGGAGTTGAAGC GAGAGTTCCAAGCAGAACAC 50 +451 -730