大豆巢式关联作图群体蛋白质含量的遗传解析

李曙光,曹永策,贺建波,王吴彬,邢光南,杨加银,赵团结,盖钧镒

Genetic Dissection of Protein Content in a Nested Association Mapping Population of Soybean

ShuGuang LI,YongCe CAO,JianBo HE,WuBin WANG,GuangNan XING,JiaYin YANG,TuanJie ZHAO,JunYi GAI

表3 大豆NAM群体中检测到的蛋白质含量QTL

Table 3 QTLs associated with the protein content detected in the soybean NAM population

QTL	染色体 Chr.	位置 Position (bp)	等位变异 No. allele	-lgP	R²(%)	SoyBase QTL^a	GWAS QTL^b
qProt-6-1	6	5723944—5861193	3	89.2	3.99	30-5, 005, 007, 015	[16-17]
qProt-7-2	7	7895394—7951423	4	54.5	2.46	33-5	[18]
qProt-20-8	20	45758806—45958325	5	52.6	2.42
qProt-19-3	19	45138372—45336506	5	39.9	1.85	2-2, 36-31	[19]
qProt-6-4	6	13682742—13879752	4	38.4	1.74	34-2	[20,17]
qProt-2-2	2	8795893—8902236	4	37.8	1.71	21-4	[20]
qProt-5-5	5	38956925—39141177	4	36.1	1.64	2-1, 9-1, 12-1, 34-1, 011, 014
qProt-18-1	18	2346289—2531952	5	31.1	1.45	26-12, 28-5, 36-25	[21]
qProt-8-5	8	31139492—31248131	5	27.6	1.30
qProt-14-2	14	6415938—6607451	3	23.2	1.02	1-6, 4-10, 45-1	[21]
qProt-13-1	13	5277987—5435217	3	22.5	0.99	36-21
qProt-1-3	1	52165879—52301103	3	21.6	0.95	36-9	[5]
qProt-4-2	4	47959591—48019889	5	19.3	0.92	4-2, 37-2	[21]
qProt-16-3	16	34706667—34803913	5	19.1	0.91	41-6
qProt-10-1	10	3262276	2	19.2	0.79	41-11	[22]
qProt-20-4	20	18696597—18710242	3	17.7	0.73	1-3, 1-4, 3-12, 10-1, 11-1, 30-1, 31-1, 36-26, 37-8, 47-8, 003	[23]
qProt-13-4	13	39868073—40038639	5	14.8	0.72	26-11
qProt-6-7	6	20701467—20900668	5	13.9	0.68	012
qProt-6-9	6	41656522—41677922	3	13.9	0.61
QTL	染色体 Chr.	位置 Position (bp)	等位变异 No. allele	-lgP	R²(%)	SoyBase QTL^a	GWAS QTL^b
qProt-7-4	7	18234251	2	14.3	0.58	47-1
qProt-6-3	6	10921339—11119531	3	12.9	0.57
qProt-14-3	14	9912397—10112026	3	12.9	0.57	21-8
qProt-9-1	9	35965266—36048472	4	12.0	0.56	5-3, 33-3, 34-6, 35-4, 36-28, 36-29, 36-30, 37-11
qProt-11-1	11	5786106—5983572	5	11.1	0.55	3-2, 16-1, 34-7, 36-2, 37-1	[20-23]
qProt-2-1	2	4025110	2	12.9	0.52		[24]
qProt-5-2	5	1708219	2	12.4	0.50
qProt-5-3	5	32642277—32673061	3	11.4	0.50	36-1
qProt-7-1	7	176227—372535	5	10.1	0.50
qProt-19-2	19	39675711—39753005	4	9.2	0.44	30-7, 36-32
qProt-20-3	20	10292622—10303142	4	9.2	0.44	1-3, 1-4, 3-12, 10-1, 11-1, 30-1, 36-26, 37-8, 47-8	[25]
qProt-6-6	6	16510997—16689830	4	9.1	0.43	21-3, 26-7, 35-1, 36-7, 36-8
qProt-10-3	10	43721962—43903254	5	8.3	0.42	27-5	[1,26]
qProt-20-6	20	34459783—34480867	4	8.6	0.41	26-5, 34-11	[26]
qProt-1-4	1	54251785—54443212	5	7.9	0.40	36-9	[22]
qProt-8-3	8	23303969—23383404	3	9.2	0.40
qProt-1-1	1	2560155—2683983	4	8.2	0.39		[21-22]
qProt-15-1	15	3194114	2	9.5	0.38	30-3	[1,16]
qProt-1-2	1	4621764—4727603	5	7.1	0.37		[21]
qProt-15-3	15	50539746—50738644	5	7.1	0.37
qProt-14-5	14	16634057—16648499	3	7.5	0.33
qProt-16-1	16	4780910	2	8.2	0.32	4-7	[6,25]
qProt-8-1	8	7846414—8000705	5	5.8	0.31	26-1, 30-4, 34-4, 34-5, 013, 016
qProt-16-5	16	37249323—37294601	4	6.5	0.31	41-6
qProt-18-4	18	51679638—51878009	4	6.2	0.30	34-9
qProt-8-6	8	46434966—46595148	3	6.5	0.28	14-1, 21-1	[25]
qProt-3-1	3	1011738	2	7.1	0.27	010
qProt-3-2	3	39567898—39600101	5	4.9	0.27	36-34, 21-9, 36-37
qProt-5-1	5	1531632—1531635	2	6.8	0.26
qProt-18-3	18	5553907—5635734	2	6.7	0.26	47-6
qProt-1-5	1	55836890—55889699	3	5.8	0.25
qProt-4-1	4	41014079	2	6.5	0.25
qProt-2-5	2	49426511—49624658	4	5.0	0.24	37-4
qProt-16-4	16	34859125—35032972	5	5.5	0.24	41-6	[27]
qProt-13-2	13	18987333-18987363	2	6.0	0.23	26-13, 36-20, 36-21, 36-23	[5]
qProt-15-2	15	4817369	2	6.1	0.23	30-3	[18,27-29]
qProt-6-11	6	48032833—48165768	4	4.4	0.22	13-2
qProt-6-2	6	6233427—6256962	2	5.6	0.21	30-5, 31-4, 005, 007, 015
QTL	染色体 Chr.	位置 Position (bp)	等位变异 No. allele	-lgP	R²(%)	SoyBase QTL^a	GWAS QTL^b
qProt-6-8	6	22700125—22889619	4	4.2	0.21
qProt-8-4	8	27091794—27092100	3	4.9	0.21
qProt-11-3	11	36538681—36538733	2	5.6	0.21	26-6
qProt-20-7	20	42051825—42200759	5	3.8	0.21
qProt-12-1	12	36587265	2	5.5	0.20	5-2, 21-10, 33-1
qProt-17-1	17	33206802—33271301	4	4.1	0.20	36-14, 36-16
qProt-6-5	6	14689566—14795226	4	3.8	0.19	34-2	[1]
qProt-6-10	6	42894533—43089859	4	3.7	0.19	24-1
qProt-14-1	14	4726017—4860851	4	3.7	0.19	1-6, 4-11, 45-1	[22]
qProt-20-2	20	5157137	2	4.5	0.16	1-3, 1-4, 3-12, 10-1, 11-1, 30-1, 36-26, 37-8, 47-8
qProt-3-3	3	44249912—44442395	3	3.4	0.15	36-35, 27-4
qProt-6-12	6	48187414	2	4.2	0.15	13-2	[20-21]
qProt-8-2	8	8219073—8363193	5	2.4	0.15	26-1, 30-4, 34-4, 34-5, 013, 016	[6,26]
qProt-14-4	14	13291266	2	4.2	0.15
qProt-18-8	18	62050016—62248618	5	2.5	0.15	30-10
qProt-20-9	20	46154443	2	4.2	0.15
qProt-3-4	3	44486103—44672295	4	3.3	0.14	36-35, 27-4
qProt-18-2	18	2963699—3158571	5	2.2	0.14	20-1, 47-6	[6]
qProt-20-5	20	33207484—33279787	3	2.9	0.13	1-1, 1-2, 15-1, 26-5, 34-11, 39-4	[30]
qProt-7-3	7	18234233	2	3.5	0.12	47-1
qProt-7-5	7	35580968—35653659	3	2.8	0.12	41-9
qProt-18-7	18	60127020—60312441	5	1.9	0.12	3-10, 30-10
qProt-2-4	2	47360405	2	3.2	0.11	40-5	[23]
qProt-11-2	11	7962963	2	3.2	0.11
qProt-16-2	16	33179205	2	3.1	0.11
qProt-13-3	13	36897211	2	3.0	0.10	6-2, 24-2
qProt-18-5	18	52822014	2	3.0	0.10	3-8, 28-2
qProt-18-6	18	54160848	2	2.9	0.10	3-9
qProt-2-3	2	12237524	2	2.6	0.09	36-12
qProt-19-1	19	37053052—37053084	2	2.4	0.08
qProt-20-1	20	1315771—1315813	2	2.5	0.08	26-3, 26-4, 37-7, 37-9, 41-4
qProt-5-4	5	38098083	2	2.1	0.07	12-1, 011, 014	[6]
qProt-10-2	10	40629142—40629143	2	1.9	0.06	27-5, 40-1
LC QTL		10	42		19.58
SC QTL		80	261		26.06
总计Total		90	303		45.64	67(119)	33(45)