藏黄牛与宣汉黄牛心脏miRNA表达谱比较
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陈露露,王会,王吉坤,王嘉博,柴志欣,陈智华,钟金城
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Comparative Analysis of miRNA Expression Profiles in the Hearts of Tibetan Cattle and Xuanhan Cattle
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LuLu CHEN,Hui WANG,JiKun WANG,JiaBo WANG,ZhiXin CHAI,ZhiHua CHEN,JinCheng ZHONG
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表2 数据过滤及去端头情况统计
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Table 2 Data filtering and de-joining statistics
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读段类型
Type of reads | 藏黄牛1
数量Count | 藏黄牛2
数量Count | 藏黄牛3
数量Count | 平均值
Average value | 宣汉黄牛1
数量Count | 宣汉黄牛2
数量Count | 宣汉黄牛3
数量Count | 平均值
Average value | 总读值
Total reads | 17274872 (100%) | 14655372 (100%) | 21634340 (100%) | 17854861 | 14186785 (100%) | 13820881 (100%) | 13651263 (100%) | 13886310 | 高质量读值
Reads with high quality | 16864234 (97.6229%) | 14331976 (97.7933%) | 21194128 (97.9652%) | 17463446 | 13970084 (98.4725%) | 13591498 (98.3403%) | 13426853
(98.3561%) | 13662812 | 3' 端缺失
Reads with null 3'adapter | 41744 (0.2475%) | 31922 (0.2227%) | 31922 (0.4363%) | 35196 | 44975 (0.3219%) | 29374 (0.2161%) | 33735 (0.2513%) | 36028 | 插入缺失读值
Reads with insert | 101492 (0.6018%) | 74047 (0.5167%) | 51722 (0.2440%) | 75754 | 108440 (0.7762%) | 72639 (0.5344%) | 93029 (0.6929%) | 91369 | 5'端污染的读值
5'adapter contaminants | 7946 (0.0471%) | 15266 (0.1065%) | 7948 (0.0375%) | 10387 | 18678 (0.1337%) | 20305 (0.1494%) | 39930 (0.2974%) | 26304 | 小于18nt的读值
Reads shorter than 18nt | 437113 (2.5920%) | 598024 (4.1727%) | 480882 (2.2689%) | 505340 | 811209 (5.8068%) | 998246 (7.3446%) | 1414065 (10.5316%) | 1074507 | 含有poly(A)的序列
Reads with polyA | 139 (0.0008%) | 133 (0.0009%) | 316 (0.0015%) | 196 | 37 (0.0003%) | 35 (0.0003%) | 59
(0.0004%) | 44 | 低频数读值(< 2)
Reads with Low frequency | 228502 (1.3550%) | 270838 (1.8897%) | 292937 (1.3822%) | 264092 | 369657 (2.6461%) | 300701 (2.2124%) | 467408 (3.4811%) | 379255 | 干净读值
Clean reads | 16047298 (95.1558%) | 13341746 (93.0908%) | 20267845 (95.6295%) | 16552296 | 12617088 (90.3150%) | 12170198 (89.5427%) | 11378627 (84.7453%) | 12055304 |
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