藏黄牛与宣汉黄牛心脏miRNA表达谱比较

陈露露,王会,王吉坤,王嘉博,柴志欣,陈智华,钟金城

Comparative Analysis of miRNA Expression Profiles in the Hearts of Tibetan Cattle and Xuanhan Cattle

LuLu CHEN,Hui WANG,JiKun WANG,JiaBo WANG,ZhiXin CHAI,ZhiHua CHEN,JinCheng ZHONG

表1 荧光定量引物

Table 1 Fluorescent quantitative primers

miRNAs miRNAs 引物序列（5'-3'） Primer sequence（5'-3'） bta-miR-99a-5p RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAAGATC F: CTGGAGAACCCGTAGATCCGAT bta-miR-100 RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAGTT F: CTGGAGAACCCGTAGATCCGAA miR-99-x RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAAGA F: CTGGAG AACCCGTAGATCCGAT bta-miR-1468 RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGCAAA F: CTGGAGGTCGTATCCAGTGCAG bta-miR-1 RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATACATCT F: CTGGAGTCGGATCCGTCTGAGC bta-miR-148a RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAAAGTT F: CTGGAGTCAGTGCACTACAGAA miR-101-y RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTCAGTT F: CTGGAGTACAGTACTGTGATAA bta-miR-499 RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAACATCA F: CTGGAGTTAAGACTTGCAGTGA miRNA统一反向引物 Unified reverse primer GTGCAGGGTCCGAGGT