猪骨骼肌卫星细胞分离培养、鉴定及其生物学特性
秦本源,杨阳,张燕伟,刘敏,张万锋,王海珍,吴怡琦,张雪莲,蔡春波,高鹏飞,郭晓红,李步高,曹果清(
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Isolation, Culture, Identification and Biological Characteristics of Pig Skeletal Muscle Satellite Cells
QIN BenYuan,YANG Yang,ZHANG YanWei,LIU Min,ZHANG WanFeng,WANG HaiZhen,WU YiQi,ZHANG XueLian,CAI ChunBo,GAO PengFei,GUO XiaoHong,LI BuGao,CAO GuoQing()
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图9. 骨骼肌卫星细胞成骨诱导分化及茜素红染色
A-C：成骨诱导7、14、21 d卫星细胞显微镜下观察（100×）;D-F：茜素红可将诱导后细胞形成的钙化结节染成红色（100×）;G-I：肉眼观察诱导后皿底出现红色沉淀结节
Fig. 9. Osteogenic differentiation of skeletal muscle satellite cells and Alizarin Red staining
A-C: After osteogenic induction 7, 14, 21 d, satellite cells were observed under microscope (100×); D-F: Alizarin red staining can stain the calcified nodules formed after induction into red (100×); G-I: Macroscopic observation of red precipitated nodules at the bottom after the osteogenesis
