猪骨骼肌卫星细胞分离培养、鉴定及其生物学特性
秦本源,杨阳,张燕伟,刘敏,张万锋,王海珍,吴怡琦,张雪莲,蔡春波,高鹏飞,郭晓红,李步高,曹果清(
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Isolation, Culture, Identification and Biological Characteristics of Pig Skeletal Muscle Satellite Cells
QIN BenYuan,YANG Yang,ZHANG YanWei,LIU Min,ZHANG WanFeng,WANG HaiZhen,WU YiQi,ZHANG XueLian,CAI ChunBo,GAO PengFei,GUO XiaoHong,LI BuGao,CAO GuoQing()
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图4. 骨骼肌卫星细胞成肌诱导分化及免疫荧光染色
A：诱导48 h,卫星细胞融合出现少量肌管;B：诱导72 h,肌管数量增多;C：诱导4 d后,肌管之间相互融合;D：分化后MHC免疫荧光;E：DAPI染核;F：D和E叠加
Fig. 4. Myogenic differentiation of skeletal muscle satellite cells and immunofluorescence (100×)
A: After 48 h induction, satellite cells fusion and a amount of myotubes were appeared; B: Induced 72 h, the number of myotubes increased; C: After 4 days induction, myotubes were fused together; D: MHC immunofluorescence; E: DAPI stained nuclei; F: Image merged between D and E
